PMID: 11607252Jan 15, 1992Paper

5'-Azido-[3,6-3H2]-1-napthylphthalamic acid, a photoactivatable probe for naphthylphthalamic acid receptor proteins from higher plants: identification of a 23-kDa protein from maize coleoptile plasma membranes

Proceedings of the National Academy of Sciences of the United States of America
R ZettlK Palme

Abstract

1-Naphthylphthalamic acid (NPA) is a specific inhibitor of polar auxin transport that blocks carrier-mediated auxin efflux from plant cells. To allow identification of the NPA receptor thought to be part of the auxin efflux carrier, we have synthesized a tritiated, photolabile NPA analogue, 5'-azido-[3,6-3H2]NPA ([3H2]N3NPA). This analogue was used to identify NPA-binding proteins in fractions highly enriched for plasma membrane vesicles isolated from maize coleoptiles (Zea mays L.). Competition studies showed that binding of [3H2]N3NPA to maize plasma membrane vesicles was blocked by nonradioactive NPA but not by benzoic acid. After incubation of plasma membrane vesicles with [3H2]N3NPA and exposure to UV light, we observed specific photoaffinity labeling of a protein with an apparent molecular mass of 23 kDa. Pretreatment of the plasma membrane vesicles with indole-3-acetic acid or with the auxin-transport inhibitors NPA and 2,3,5-triiodobenzoic acid strongly reduced specific labeling of this protein. This 23-kDa protein was also labeled by addition of 5-azido-[7-3H]indole-3-acetic acid to plasma membranes prior to exposure to UV light. The 23-kDa protein was solubilized from plasma membranes by 1% Triton X-100. The possibili...Continue Reading

References

May 7, 1991·Biochimica Et Biophysica Acta·H FelleK Palme
Jan 1, 1989·Analytical Biochemistry·T Reinard, H J Jacobsen
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G R HicksT L Lomax
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J FeldwischK Palme
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Citations

Dec 1, 1994·Plant Molecular Biology·R Hooley
Jan 1, 1994·Protoplasma·B G Pickard
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J FeldwischK Palme
Nov 4, 2016·Biological Procedures Online·Mujahid IqbalZonghui Yuan
Oct 29, 1992·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R Hedrich, A Jeromin
Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Estelle
Jun 16, 2010·Journal of the Science of Food and Agriculture·Oscar Aguilar, Marco Rito-Palomares
Feb 1, 1995·The New Phytologist·Richard M Napier, Michael A Venis
Aug 16, 1996·Science·M J BennettK A Feldmann

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