5-Bromodeoxyuridine suppresses position effect variegation of transgenes in HeLa cells

Experimental Cell Research
T SuzukiDai Ayusawa

Abstract

An ectopic gene integrated in the host genome is occasionally silenced due to a position effect of its adjacent chromatin structure. We found that 5-bromodeoxyuridine clearly activated such a transgene in HeLa cells. The transgene was also activated to various degrees by inhibitors of histone deacetylase, DNA topoisomerases, or DNA methyltransferase. The peptide antibiotic distamycin A potentiated markedly the effect of 5-bromodeoxyuridine. Transient expression of an artificial AT-hook protein termed MATH20 also potentiated its effect although significantly activated the transgene alone. Since distamycin A and MATH20 are able to displace histone H1 and other DNA-binding proteins bound to specific AT-rich sequences by a dominant, mutually exclusive fashion, these results suggest that 5-bromodeoxyuridine targets such an AT-rich sequence located adjacent to the silenced transgene, resulting in chromatin accessibility.

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Citations

Apr 11, 2007·Bioscience, Biotechnology, and Biochemistry·Morio EndohDai Ayusawa
May 31, 2002·Bioscience, Biotechnology, and Biochemistry·Eriko MichishitaDai Ayusawa
Jul 9, 2004·Experimental Gerontology·Sachi MinagawaDai Ayusawa
Feb 9, 2008·Biochemical and Biophysical Research Communications·Kensuke MikiDai Ayusawa
Nov 19, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J C de AlmeidaJ C Viana
Jun 18, 2003·Microscopy Research and Technique·Francine Puvion-DutilleulOlivier Albagli-Curiel
Mar 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryo UkekawaDai Ayusawa
Mar 31, 2010·Molecular Genetics and Genomics : MGG·Michihiko FujiiDai Ayusawa
Mar 14, 2003·Critical Reviews in Oncology/hematology·Vladimir N Anisimov

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