5α-dihydroprogesterone concentrations and synthesis in non-pregnant mares

The Journal of Endocrinology
A J ConleyS D Stanley

Abstract

In vivo and in vitro evidence indicates that the bioactive, 5α-reduced progesterone metabolite, 5α-dihydroprogesterone (DHP) is synthesized in the placenta, supporting equine pregnancy, but its appearance in early pregnancy argues for other sites of synthesis also. It remains unknown if DHP circulates at relevant concentrations in cyclic mares and, if so, does synthesis involve the non-pregnant uterus? Jugular blood was drawn daily from cyclic mares (n = 5). Additionally, ovariectomized mares (OVX) and geldings were administered progesterone (300 mg) intramuscularly. Blood was drawn before and after treatment. Incubations of whole equine blood and hepatic microsomes with progesterone were also investigated for evidence of DHP synthesis. Sample analysis for progesterone, DHP and other steroids employed validated liquid chromatography-tandem mass spectrometry methods. Progesterone and DHP appeared a day (d) after ovulation in cyclic mares, was increased significantly by d3, peaking from d5 to 10 and decreased from d13 to 17. DHP was 55.5 ± 3.2% of progesterone concentrations throughout the cycle and was highly correlated with it. DHP was detected immediately after progesterone administration to OVX mares and geldings, maintaining...Continue Reading

References

Mar 1, 1979·Journal of Reproduction and Fertility·D P NeelyC L Sauter
Jul 1, 1991·Journal of Clinical Pathology·F V Flynn
Jul 1, 1991·General and Comparative Endocrinology·D W MakawitiS Gombe
Jun 1, 1991·Journal of Clinical Pathology·F P RugmanC R Hay
Apr 1, 1972·Journal of Reproduction and Fertility·J Hanks, R V Short
Dec 1, 1971·American Journal of Obstetrics and Gynecology·I H ThorneycroftR M Nakamura
Apr 28, 2001·Life Sciences·S KirkmanH C Potgieter
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth L ScholtzAlan J Conley
Jan 28, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Erin L LegackiAlan J Conley
Jul 29, 2016·The Journal of Endocrinology·C J CorbinA J Conley
Oct 27, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Erin L LegackiAlan J Conley

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Citations

Feb 15, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·Alan J ConleyLawrence P Reynolds

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