PMID: 9419337Feb 21, 1998Paper

5' end maturation and RNA editing have to precede tRNA 3' processing in plant mitochondria

Proceedings of the National Academy of Sciences of the United States of America
A KunzmannA Marchfelder

Abstract

We report the characterization and partial purification of potato mitochondrial RNase Z, an endonuclease that generates mature tRNA 3' ends. The enzyme consists of one (or more) protein(s) without RNA subunits. Products of the processing reaction are tRNA molecules with 3' terminal hydroxyl groups and 3' trailers with 5' terminal phosphates. The main processing sites are located immediately 3' to the discriminator and one nucleotide further downstream. This endonucleolytic processing at and close to the tRNA 3' end in potato mitochondria suggests a higher similarity to the eukaryotic than to the prokaryotic tRNA 3' processing pathway. Partial purification and separation of RNase Z from the 5' processing activity RNase P allowed us to determine biochemical characteristics of the enzyme. The activity is stable over broad pH and temperature ranges, with peak activity at pH 8 and 30 degrees C. Optimal concentrations for MgCl2 and KCl are 5 mM and 30 mM, respectively. The potato mitochondrial RNase Z accepts only tRNA precursors with mature 5' ends. The precursor for tRNAPhe requires RNA editing for efficient processing by RNase Z.

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Citations

Sep 13, 2005·Journal of Molecular Evolution·Hyosig Won, Susanne S Renner
Jun 17, 1999·FEMS Microbiology Reviews·A Schön
Apr 12, 2002·Gene·J FeyL Maréchal-Drouard
Jan 18, 2005·Nature·Inés Li de la Sierra-GallayCiarán Condon
Mar 17, 2007·Nature Reviews. Microbiology·Yulia RedkoCiarán Condon
Mar 7, 2006·Nature Structural & Molecular Biology·Inés Li de la Sierra-GallayCiarán Condon
Sep 10, 2010·The Journal of Biological Chemistry·Susan FischerAnita Marchfelder
Mar 17, 2001·EMBO Reports·M Mörl, A Marchfelder
May 29, 2002·The EMBO Journal·Steffen SchifferAnita Marchfelder
Jan 13, 2004·Nucleic Acids Research·Edward B DubrovskyAnita Marchfelder
Oct 13, 2004·Nucleic Acids Research·Louis LevingerCatherine Florentz
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stefan Binder, Axel Brennicke
May 5, 2009·Plant Physiology·Giusy CaninoAnita Marchfelder
Oct 26, 2011·Biochemistry. Biokhimii︠a︡·B Castandet, A Araya
Jan 30, 2003·RNA·Joanna Kufel, David Tollervey
Oct 11, 2001·Biological Chemistry·H SchürerM Mörl
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Feb 9, 2005·The Journal of Biological Chemistry·Oliver SchillingAndreas Vogel
Aug 3, 2005·The Journal of Biological Chemistry·Antonio PlacidoLaurence Maréchal-Drouard
Aug 25, 2005·The Journal of Biological Chemistry·Bettina SpäthAnita Marchfelder
Sep 20, 2000·The Journal of Biological Chemistry·S T KapushocL Simpson
Jul 30, 2005·The Journal of Biological Chemistry·María Ceballos-Chávez, Agustín Vioque
Dec 11, 2020·Plant Physiology and Biochemistry : PPB·Guoqing PengHai Zhou
Mar 9, 2002·Journal of Molecular Biology·Karina SchierlingAnita Marchfelder
Nov 20, 2013·RNA·Ewa SkowronekJoanna Kufel

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