5'-end maturation of tRNA in aquifex aeolicus

Biological Chemistry
Michal MarszalkowskiRoland K Hartmann

Abstract

5'-End maturation of tRNA primary transcripts is thought to be ubiquitously catalyzed by ribonuclease P (RNase P), a ribonucleoprotein enzyme in the vast majority of organisms and organelles. In the hyperthermophilic bacterium Aquifex aeolicus, neither a gene for the RNA nor the protein component of bacterial RNase P has been identified in its sequenced genome. Here, we demonstrate the presence of an RNase P-like activity in cell lysates of A. aeolicus. Detection of activity was sensitive to the buffer conditions during cell lysis and partial purification, explaining why we failed to observe activity in the buffer system applied previously. RNase P-like activity of A. aeolicus depends on the presence of Mg2+ or Mn2+, persists at high temperatures, which inactivate RNase P enzymes from mesophilic bacteria, and is remarkably resistant to micrococcal nuclease treatment. While cellular RNA fractions from other Aquificales (A. pyrophilus, Hydrogenobacter thermophilus and Thermocrinis ruber) could be stimulated by bacterial RNase P proteins to catalyze tRNA 5'-end maturation, no such stimulation was observed with RNA from A. aeolicus. In conclusion, our results point to the possibility that RNase P-like activity in A. aeolicus is dev...Continue Reading

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Citations

Dec 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lien B LaiTodd M Lowe
Jul 16, 2010·BMC Genomics·Dilmurat YusufIvo L Hofacker
Feb 21, 2016·Nucleic Acids Research·Nadia BrillanteRoland K Hartmann
Jan 20, 2016·Genome Biology and Evolution·Dorota MatelskaStanislaw Dunin-Horkawicz
May 19, 2012·IUBMB Life·Aaron Hernandez-CidAlfredo Torres-Larios
Dec 6, 2011·Biochimica Et Biophysica Acta·Walter Rossmanith
Jul 28, 2010·FEMS Microbiology Reviews·Cecília M ArraianoSandra C Viegas
Feb 14, 2009·Progress in Molecular Biology and Translational Science·Roland K HartmannAnita Marchfelder
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Astrid I NickelRoland K Hartmann
Jun 29, 2021·ELife·Rebecca FeyhFlorian Altegoer

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