5-HT control of ischemia-evoked glutamate efflux from human cerebrocortical slices

Neurochemistry International
Manuela MarcoliGuido Maura

Abstract

The aim of the present investigation was to explore if glutamate efflux from human cerebrocortical slices caused by oxygen/glucose deprivation can be controlled by 5-hydroxytryptamine (5-HT). Slices were superfused in aerobic conditions or in conditions simulating moderate ischemic insult (24 min oxygen and glucose deprivation) and the efflux of previously accumulated [3H]D-aspartate and of endogenous glutamate was measured in superfusate fractions. The efflux of both [3H]D-aspartate and endogenous glutamate evoked by ischemia were reduced by at least 50% in the presence of 5-HT (1 microM). Moreover, the 5-HT(1A) receptor agonist 8-OH-DPAT (1 microM) mimicked the 5-HT effect. We conclude that activation of 5-HT receptors of the 5-HT(1A) subtype might help to control glutamate efflux and excitotoxic damage during ischemia in human cerebral cortex and would deserve to be considered in a multipharmacological approach to neuroprotection in brain ischemia.

Citations

May 19, 2009·Neuroscience Bulletin·Zhao-Hui GuoWei-Zhi Wang
Mar 3, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Cletus CheyuoPing Wang
Mar 16, 2012·Journal of Neuroscience Research·Teruyuki HirakiJoel H Greenberg
May 23, 2006·Pharmacological Reviews·Maurizio Raiteri
Aug 25, 2019·Neurochemistry International·Jingxi MaZhiyou Cai

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