5-HT1A, 5-HT2, and GABAB receptors interact to modulate neurotransmitter release probability in layer 2/3 somatosensory rat cortex as evaluated by the paired pulse protocol

Journal of Neuroscience Research
José L Torres-EscalanteJuan C Pineda

Abstract

Activation of gamma-aminobutyric acid B (GABA(B)) and 5-hydroxytryptamine (5-HT) receptors produces presynaptic inhibition at glutamatergic terminals in the rat neocortex. To evaluate interactions between these metabotropic receptors, field potentials were recorded in layer 2/3 of somatosensory cortex. In addition, the paired pulse (PP) protocol was used to measure changes in the ratio of the second/first extracellular synaptic potentials (S(2)/S(1) ratio) as an index of glutamate release probability in the area. Lowering extracellular [Ca(2+)](o) to 0.5 mM, increased the S(2)/S(1) ratio by 318 +/- 134%. 5-HT (1 microM) increased the S(2)/S(1) ratio by 61 +/- 15%. In presence of the GABA(A) antagonist bicuculline (10 microM), 5-HT increased the S(2)/S(1) ratio by 98 +/- 15%. This effect did not desensitize after two consecutive applications of the amine, and was dose dependent in the concentration range between 0.03-1 microM (EC(50) = 2.36 x 10(-7) mol/L). The increase of S(2)/S(1) ratio induced by 5-HT (1 microM) was blocked reversibly by the 5-HT(1A) antagonist NAN-190 (10-30 microM), but was unaffected by the selective GABA(B) antagonist CGP 52432 (1 microM). The action of 5-HT was mimicked by the 5-HT(1A/7) agonist 8OH-DPAT...Continue Reading

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Citations

Sep 15, 2010·Pflügers Archiv : European journal of physiology·José A Troca-Marín, Emilio Geijo-Barrientos
Nov 15, 2011·Molecular Neurobiology·M Victoria Puig, Allan T Gulledge
Oct 15, 2013·Cephalalgia : an International Journal of Headache·Domenico RestucciaSergio Zanini
Apr 12, 2013·Cephalalgia : an International Journal of Headache·Domenico RestucciaSergio Zanini
Oct 11, 2005·Pain·Massimiliano ValerianiFederico Vigevano
May 14, 2020·Journal of Neurophysiology·Tyler R SizemoreAndrew M Dacks
Dec 6, 2006·The Journal of Biological Chemistry·Srividya BalasubramanianRandy A Hall

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