5-HT1A and 5-HT2 receptors differentially regulate the excitability of 5-HT-containing neurones of the guinea pig dorsal raphe nucleus in vitro

Brain Research
R CravenN Newberry

Abstract

We have used intracellular recording techniques to examine the effects of 5-hydroxytryptamine (5-HT, serotonin) on 5-HT-containing neurones of the guinea pig dorsal raphe nucleus in vitro. Bath-applied 5-HT (30-300 microM) had two opposing effects on the membrane excitability of these cells, reflecting the activation of distinct 5-HT receptor subtypes. As demonstrated previously in the rat, 5-HT evoked a hyperpolarization and inhibition of 5-HT neurones, which appeared to involve the activation of an inwardly rectifying K(+) conductance. This hyperpolarizing response was blocked by the 5-HT(1A) receptor-selective antagonist WAY-100635 (30-100 nM). In the presence of WAY-100635, 5-HT induced a previously unreported depolarizing, excitatory response of these cells, which was often associated with an increase in the apparent input resistance of the neurone, likely due to the suppression of a K(+) conductance. Like the hyperpolarizing response to 5-HT, this depolarization could be recorded in the presence of the Na(+) channel blocker tetrodotoxin. In addition, the response was not significantly attenuated by the alpha(1)-adrenoceptor antagonist prazosin (500 nM), indicating that it is not due to the release of noradrenaline, or to ...Continue Reading

Citations

Jan 1, 2013·The Journal of Physiological Sciences : JPS·Yoshihiro GochoFumihito Saitow
Mar 22, 2013·Journal of Neurophysiology·Hyo-Jin JeongChristopher W Vaughan
Jul 16, 2004·Journal of Neurophysiology·Silvia MarinelliChristopher W Vaughan
Dec 14, 2011·Molecular Psychiatry·S L DiazL Maroteaux
Jan 9, 2019·Frontiers in Neuroscience·Emily QuentinLuc Maroteaux
Feb 18, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Arnauld BelmerLuc Maroteaux
Jan 24, 2012·Physiology & Behavior·Mateus Dalbem Ferreira, Leda Menescal-de-Oliveira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.