PMID: 9448942Feb 4, 1998Paper

5-HT3 receptors induce rises in cytosolic and nuclear calcium in NG108-15 cells via calcium-induced calcium release

Cell Calcium
P Rondé, R A Nichols

Abstract

A complete understanding of how excitatory ligand-gated ion channels regulate intracellular Ca2+ in nerve cells remains to be elucidated. Laser-scanning confocal microscopy was used here to measure Ca2+ changes in the neuroblastoma x glioma hybrid cell line NG108-15, employed as a model nerve cell line, upon activation by the 5-HT3 receptor, a serotonin-activated ligand-gated ion channel. Addition of the 5-HT3 agonist 1-m-(chlorophenyl)-biguanide (mCPBG) induced increases in [Ca2+]i in both the cytoplasm and the nuclei of the NG108-15 cells. Using high-time resolution line scanning, no delay was evident between the mCPBG-induced rise in cytosolic [Ca2+]i and the rise in nuclear [Ca2+]i. The agonist-induced responses were completely blocked by addition of EGTA to chelate external Ca2+ and by addition of the 5-HT3 receptor antagonist tropisetron or the L-type Ca2+ channel blocker nitrendipine. Caffeine, but not thapsigargin, treatment significantly reduced the mCPBG-induced responses in the nucleus and the cytoplasm, both to the same extent. We conclude that, upon 5-HT3 receptor activation, Ca2+ enters the cells through voltage-gated Ca2+ channels and then triggers the release of Ca2+ from ryanodine-sensitive intracellular stores...Continue Reading

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Citations

Apr 23, 2003·Trends in Pharmacological Sciences·Johannes A van Hooft, Jerrel L Yakel
Apr 27, 1999·Brain Research. Molecular Brain Research·A D SpierS C Lummis
Feb 13, 1999·Progress in Neurobiology·M J Millan
Jan 1, 2007·The Libyan Journal of Medicine·Sm AburawiZm Gorash
Mar 19, 2014·Pharmacology, Biochemistry, and Behavior·Weixia ZhongNissar A Darmani
Mar 20, 2015·PloS One·Bingjin LiRanji Cui
Jul 26, 2007·The European Journal of Neuroscience·Edith DoucetMichel B Emerit
Jul 29, 2005·Journal of Neurophysiology·Pierre A Guertin, Inge Steuer
Mar 30, 2007·Molecular Pharmacology·Beate NieslerMichael Brüss
Jun 3, 2021·International Journal of Molecular Sciences·Weixia ZhongNissar A Darmani
Jul 1, 2004·Journal of Cell Science·Oleg Gerasimenko, Julia Gerasimenko

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