PMID: 9435249Jan 22, 1998Paper

5-Lipoxygenase expression in Langerhans cells of normal human epidermis

Proceedings of the National Academy of Sciences of the United States of America
R SpanbroekA J Habenicht

Abstract

We studied expression of the 5-lipoxygenase (5-LO) pathway in normal human skin. In situ hybridization revealed a 5-LO mRNA-containing epidermal cell (EC) population that was predominantly located in the midportion of the spinous layer, in outer hair root sheaths, and in the epithelial compartment of sebaceous glands. Examination of skin specimens by immunohistochemistry and of primary ECs by flow cytometry mapped the 5-LO protein exclusively to Langerhans cells (LCs). The LC 5-LO protein was largely found in the nuclear matrix, in nuclear envelopes, and perinuclear regions as indicated by in situ confocal laser scan microscopy. Reverse transcription-PCR and immunoblot analyses of purified primary EC populations further indicated that LCs are major 5-LO expressing cells. Enriched primary LCs were also found to contain 5-LO activating protein (FLAP), leukotriene (LT) C4 synthase, and LTA4 hydrolase. By contrast, 5-LO, FLAP, and LTC4 synthase were undetectable or largely reduced, but LTA4 hydrolase transcripts and protein were identified in ECs depleted of LCs. These data show that naive LCs are major, and possibly the sole, 5-LO pathway expressing cells in the normal human epidermis.

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Citations

Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Olof Rådmark
Apr 30, 2011·Chemical Reviews·Motonao Nakamura, Takao Shimizu
Sep 6, 2000·International Journal of Dermatology·Y F MahéB A Bernard
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Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·M Peters-Golden, T G Brock
Jan 13, 2009·BMC Immunology·Yilmaz MahshidHans-Erik Claesson
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·R SpanbroekA J Habenicht
Feb 18, 2014·Prostaglandins & Other Lipid Mediators·Dipti MangalLawrence R Soma
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rainer SpanbroekAndreas J R Habenicht
Aug 21, 2013·Biochimica Et Biophysica Acta·Peter Krieg, Gerhard Fürstenberger
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M K ChurchG F Clough
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