5-Methoxysalicylic acid and spermine: a new matrix for the matrix-assisted laser desorption/ionization mass spectrometry analysis of oligonucleotides

Journal of the American Society for Mass Spectrometry
A M Distler, J Allison

Abstract

5-Methoxysalicylic acid (MSA) is demonstrated to be a useful matrix for matrix-assisted laser desorption/ionization time-of-flight (TOF) mass spectrometry of oligonucleotides, when desorption/ionization without fragmentation is desired. When MSA is combined with the additive spermine, the need for desalting is reduced. The MSA/spermine matrix yields linear TOF mass spectra with improved resolution, less fragmentation, and less intense alkali ion adduct peaks than those spectra obtained using 3-hydroxypicolinic acid and 6-aza-2-thiothymine with spermine or diammonium hydrogen citrate as additives. Instrumental conditions are discussed to improve the spectral resolution, specifically the use of longer delay times in the delayed-extraction ion source.

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Citations

Jun 25, 2013·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Bradley T BurcarLinda B McGown
Nov 11, 2006·Journal of the American Society for Mass Spectrometry·Peran TerrierWilliam Buchmann
Sep 29, 2007·Journal of the American Society for Mass Spectrometry·Peran TerrierWilliam Buchmann
Sep 27, 2002·Journal of the American Society for Mass Spectrometry·Anne M Distler, John Allison
Feb 1, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nariyasu Mano, Junichi Goto
Jul 23, 2003·Rapid Communications in Mass Spectrometry : RCM·Fenhong Song
Oct 7, 2003·Mass Spectrometry Reviews·Yingda XuJ Throck Watson
Feb 18, 2006·Trends in Biochemical Sciences·Benjamin Thomas, Alexandre V Akoulitchev
Oct 7, 2003·Mass Spectrometry Reviews·Simone Cristoni, Luigi Rossi Bernardi
Mar 5, 2010·Mass Spectrometry Reviews·Michael B Beverly
Aug 28, 2001·Journal of Mass Spectrometry : JMS
Jun 20, 2009·Bioorganicheskaia khimiia·E N Il'ina, V M Govorun
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·Yu FuHanfa Zou

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