PMID: 118001Nov 1, 1979Paper

5'-Methylthioadenosine phosphorylase from Caldariella acidophila. Purification and properties

European Journal of Biochemistry
M Carteni'-FarinaV Zappia


The occurrence of 5'-methylthioadenosine phosphorylase in Caldariella acidophila, a thermophilic bacterium growing optimally at 87 degrees C, is reported. It represents the first example in prokaryotes of a phosphoryolytic cleavage of the thioether. The reaction products, purified by ion-exchange chromatography, have been identified as 5-methylthioribose-1-phosphate and adenine by several analytical procedures. The enzyme has been purified to homogeneity in 32% yield by using DEAE-cellulose and hydroxyapatite chromatography, gel filtration and isoelectric focusing. The enzyme shows a high degree of thermophilicity, its temperature optimum being at 93 degrees C; furthermore no loss of activity is observable after exposure for 1 h at 100 degrees C. The kinetic data indicate a sequential mechanism of the reaction. The apparent Km values are 0.095 mM for 5'-methylthioadenosine and 6.1 mM for phosphate. The specificity of the reaction is rather strict. Experiments performed with analogues of the substrate, i.e. 5'-methylthioinosine, 5'-dimethylthioadenosine sulfonium salt, 5'-n-butylthioadenosine, 5'-isobutylthioadenosine, 5'-isobutylthioinosine, adenosylhomocysteine, 5'-thioethanoladenosine, adenosine, indicate the relevance of the...Continue Reading


Feb 18, 1976·Journal of the American Chemical Society·J K CowardO Takagi
Jul 14, 1978·Biochemical and Biophysical Research Communications·J I Toohey
Dec 1, 1978·The Biochemical Journal·V ZappiaM Carteni-Farina
Jan 1, 1975·Journal of General Microbiology·M De RosaJ D Bu'lock
May 14, 1979·Biochemical and Biophysical Research Communications·L Rosa
Jan 1, 1978·The International Journal of Biochemistry·G CacciapuotiV Zappia
Oct 15, 1978·The Biochemical Journal·M De RosaV Zappia
May 1, 1977·Analytical Biochemistry·V ZappiaA de Santis
May 1, 1966·Analytical Biochemistry·S K Shapiro, D J EHNINGER
Mar 1, 1974·Analytical Biochemistry·V ZappiaL Servillo
Nov 1, 1969·The Biochemical Journal·A E Pegg, H G Williams-Ashman
Jan 1, 1971·Archives of Biochemistry and Biophysics·F SCHLENKN K Hutson
May 10, 1968·Biochemical and Biophysical Research Communications·F SCHLENK, C R Zydek
Jul 1, 1952·Archives of Biochemistry and Biophysics·R L SMITH, F SCHLENK
Jul 20, 1964·Archives of Biochemistry and Biophysics·F SCHLENK, D J EHNINGER
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS


Oct 24, 2007·Antimicrobial Agents and Chemotherapy·Janice R SufrinC J Bacchi
Jan 29, 2016·Molecular & Cellular Proteomics : MCP·Emilie Bigaud, Fernando J Corrales
Oct 12, 2018·Microbial Biotechnology·Agnieszka SekowskaAntoine Danchin

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