PMID: 114225Sep 12, 1979Paper

5-methylthioribose 1-phosphate: a product of partially purified, rat liver 5'-methylthioadenosine phosphorylase activity

Biochimica Et Biophysica Acta
A J FerroJ A Nicolette


5'-Methylthioadenosine phosphorylase from rat liver has been purified 112-fold. A molecular weight of 90 000 for the enzyme was estimated from gel filtration on Sephadex G-150. The Km for 5'-methylthioadenosine was 4.7 . 10(-7) M, while the Km for phosphate was 2 . 10(-4) M. The products of the reaction were isolated and identified as adenine and 5-methylthioribose 1-phosphate. In addition to 5'-methylthioadenosine the nucleoside analogues 5'-ethylthioadenosine and 5'-n-propylthioadenosine also served as substrates for the enzyme. The 7-deaza analogue 5'-methylthiotubercidin was found to be an inhibitor of the reaction, but was inactive as a substrate.


Dec 6, 1976·Biochemical and Biophysical Research Communications·R E LawA J Ferro
Sep 5, 1973·Biochemical and Biophysical Research Communications·C Baxter, C J Coscia
Jan 1, 1971·Archives of Biochemistry and Biophysics·F SchlenkN K Hutson
Jan 1, 1964·Medicina Experimentalis. International Journal of Experimental Medicine·J B RHODES, H G Williams-Ashman
Aug 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·M GOLDJ HURWITZ
Dec 31, 1949·Nature·C S HANES, F A ISHERWOOD


Mar 14, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·A SahotaT Gibson
Dec 18, 2001·The Biochemical Journal·Matthew J Pugmire, Steven E Ealick
May 1, 1990·Journal of Cellular Physiology·J BlancoR Franco

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