PMID: 9646951Jul 1, 1998Paper

5'-nucleotidase activity in lymphocytes from patients affected by B-cell chronic lymphocytic leukemia

Clinical Biochemistry
F RosiR Pagani

Abstract

The activity of membrane-bound ecto-5'-nucleotidase and soluble e-Ns and c-N-II 5'-nucleotidases was evaluated on lymphocytes from patients affected by B-cell chronic lymphocytic leukemia (B-CLL). A statistically significative decrease in ecto-5'-nucleotidase, e-Ns, and c-N-II activities was observed in peripheral blood lymphocytes and in B and T populations from affected individuals. For the assay of ecto-5'-nucleotidase, e-Ns, and c-N-II activity we used a radioactive procedure coupled to HPLC. Since the ecto-5'-nucleotidase is identified as CD73 antigen, we performed immunofluorescence analysis using a specific monoclonal antibody. We analyzed ecto-5'-nucleotidase mRNA by RT-PCR to ascertain the possibility of an alteration in the transcription of its gene. A decrease in ecto-5'-nucleotidase activity was correlated to reduction in ecto-5'-nucleotidase positive cells (CD73+) in leukemia patients. RT-PCR produced a fragment of the expected size and the specific mRNA was found expressed in both healthy subjects and leukemia patients. The decrease in ecto-5'-nucleotidase activity in patients with B-CLL is not due to loss of transcription of the specific mRNA. The presence of point mutations, splicing alteration, or posttranslati...Continue Reading

Citations

Jun 21, 2005·Pharmacology & Therapeutics·Sally Anne HunsuckerJozef Spychala
Aug 31, 2001·European Journal of Pharmacology·A D ConigraveJ S Wiley
May 10, 2002·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·F RosiA Tabucchi
Nov 17, 2009·Journal of Cancer Research and Clinical Oncology·Panneerselvam JananiChandrakesan Parthasarathy
Mar 13, 2009·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·F CarlucciA Tabucchi
Aug 12, 2000·Redox Report : Communications in Free Radical Research·A H ChalmersA H Winefield
Aug 6, 2011·Clinical Lymphoma, Myeloma & Leukemia·Dianne PulteAaron J Marcus
Aug 15, 2003·Leukemia & Lymphoma·Carlos M GalmariniCharles Dumontet
Oct 27, 2006·Nucleosides, Nucleotides & Nucleic Acids·R SadejA C Skladanowski
May 24, 2006·Melanoma Research·Rafal SadejAndrzej C Skladanowski
Sep 13, 2012·Experimental and Therapeutic Medicine·Guo ChenQing Xu
Sep 24, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Veena Singh GhalautRakesh Dhankhar

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