5-series peptido-leukotriene synthesis in mouse peritoneal macrophages: modulation by dietary n-3 fatty acids

Biochemical and Biophysical Research Communications
R S ChapkinK L Erickson

Abstract

This study was undertaken to elucidate the metabolic fate of dietary eicosapentaenoic acid (20:5n-3), a major n-3 polyunsaturated fatty acid constituent of fish oil, in the mouse peritoneal macrophage. Mice were fed diets containing menhaden fish oil (FO) or safflower oil (SO). After 3 weeks, resident or responsive peritoneal macrophages were isolated and stimulated with zymosan and A23187, respectively. We demonstrate the novel synthesis of leukotriene C5 (LTC5), derived from 20:5n-3, in addition to LTC4 in both resident and responsive macrophages from FO fed mice. The structures of these peptido-leukotrienes were characterized by their retention times on reverse phase high pressure liquid chromatography, by their u.v. absorbance spectra, and by cross-reactivity with peptido-leukotriene antibody. These results demonstrate for the first time that macrophages are capable of metabolizing dietary 20:5n-3 to LTC5, a pentaene peptido-leukotriene.

References

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Sep 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G Feuerstein, J M Hallenbeck
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Citations

Oct 1, 1991·Biochimica Et Biophysica Acta·R S Chapkin, K J Coble
Jan 1, 1997·Advances in Enzyme Regulation·P C Calder
Jan 29, 2000·Immunology and Cell Biology·F A WallaceP C Calder
Jul 10, 1998·The Proceedings of the Nutrition Society·E A Miles, P C Calder
Apr 9, 2004·The Proceedings of the Nutrition Society·Parveen Yaqoob
Aug 29, 2009·Journal of Atherosclerosis and Thrombosis·Shinji TakaiMizuo Miyazaki
Oct 20, 2005·Clinical and Experimental Immunology·S C MillsS C Knight
Oct 26, 2000·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·P C Calder, E A Miles

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