PMID: 2498476Feb 1, 1989Paper

6,4,4'-trimethylangelicin photoadduct formation in DNA: production and characterization of a specific monoclonal antibody

Journal of Photochemistry and Photobiology. B, Biology
G MioloF P Gasparro

Abstract

The photochemical reactions of 6,4,4'-trimethylangelicin (TMA) with calf thymus DNA and an octanucleotide containing a single thymine have been characterized. HPLC analyses of enzymatically hydrolyzed TMA-DNA showed that isomeric forms of 4',5'-furan-side monoadducts were the major products. To develop monoclonal antibodies Balb c mice were immunized with the TMA-DNA complexed with methylated bovine serum albumin. The resultant antibodies were characterized by enzyme-linked immunosorbent assays (ELISA). The most sensitive antibody (7E3) has high specificity for TMA-DNA, very low cross-reactivity with DNA modified with either 4',5'-dimethylangelicin or 4'-methylangelicin and no cross-reactivity with non-modified DNA or with DNA modified with either 4'-aminomethyl-4,5',8-trimethylpsoralen or 8-methoxypsoralen. To characterize further this antibody, oligonucleotides containing specific TMA photoadducts were isolated from the photoreaction mixture by polyacrylamide gel electrophoresis and used as competitive inhibitors in the ELISA. Autoradiography of the gel showed an intense band corresponding to the 4',5'-monoadduct and two weaker unidentified bands. Antibody 7E3 reacted only with the 4',5'-monoadduct band as would be expected s...Continue Reading

References

Feb 5, 1987·The New England Journal of Medicine·R EdelsonG Plewig
Apr 11, 1985·Nucleic Acids Research·R M SantellaR L Edelson
Dec 5, 1974·The New England Journal of Medicine·J A ParrishM A Pathak
Jul 1, 1984·Photochemistry and Photobiology·H GamperJ E Hearst

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Citations

Feb 1, 1989·Journal of Photochemistry and Photobiology. B, Biology·G MioloF Zacchello
Jun 1, 1990·Journal of Photochemistry and Photobiology. B, Biology·J CadetW R Midden
Nov 1, 1994·Journal of Photochemistry and Photobiology. B, Biology·F BordinF Baccichetti
Jun 17, 1999·Il Farmaco·G MioloF Dall'Acqua
Apr 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·S RoussetE Moustacchi
Aug 1, 1995·Photodermatology, Photoimmunology & Photomedicine·L A Amici, F P Gasparro
Dec 1, 1989·Photochemistry and Photobiology·D Averbeck
Aug 1, 1990·Photochemistry and Photobiology·F P GasparroR L Edelson
May 1, 1996·Photochemistry and Photobiology·F P Gasparro
Sep 15, 1992·Journal of Photochemistry and Photobiology. B, Biology·J CadetL Voituriez

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