6,6'-Bieckol protects insulinoma cells against high glucose-induced glucotoxicity by reducing oxidative stress and apoptosis

Fitoterapia
Mi Hwa ParkSeung-Hong Lee

Abstract

Pancreatic β cells are highly sensitive to oxidative stress, which might play an important role in β cell death in diabetes. The protective effect of 6,6'-bieckol, a phlorotannin polyphenol compound purified from Ecklonia cava, against high glucose-induced glucotoxicity was investigated in rat insulinoma cells. High glucose (30 mM) treatment induced the death of rat insulinoma cells, but treatment with 10 or 50 μg/mL 6,6'-bieckol significantly inhibited the high glucose-induced glucotoxicity. Furthermore, treatment with 6,6'-bieckol dose-dependently reduced the level of thiobarbituric acid reactive substances, generation of intracellular reactive oxygen species, and the level of nitric oxide, all of which were increased by high glucose concentration. In addition, 6,6'-bieckol protected rat insulinoma cells from apoptosis under high-glucose conditions. These effects were associated with increased expression of the anti-apoptotic protein Bcl-2 and reduced expression of the pro-apoptotic protein Bax. These findings indicate that 6,6'-bieckol could be used as a potential nutraceutical agent offering protection against the glucotoxicity caused by hyperglycemia-induced oxidative stress associated with diabetes.

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
May 29, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ilham SeghrouchniAndré Revol
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Mi-Jeong AhnJinwoong Kim
Jun 19, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Takako YokozawaGen-Ichiro Nonaka
Aug 12, 2008·Bioorganic & Medicinal Chemistry·Murat ArtanSe-Kwon Kim
Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Soo-Jin HeoYou-Jin Jeon
Nov 10, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Seung-Hong LeeYou-Jin Jeon
Jul 6, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Seung-Hong LeeYou-Jin Jeon
Jan 10, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Seung-Hong LeeYou-Jin Jeon

❮ Previous
Next ❯

Citations

Jan 4, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Graciliana LopesPatrícia Valentão
Aug 1, 2018·Marine Drugs·Manuel Gómez-GuzmánJulio Gálvez
Aug 26, 2017·Archives of Pharmacal Research·Prashamsa KoiralaJae Sue Choi
Jul 30, 2020·Marine Drugs·João CotasLeonel Pereira
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hyun-Ah LeeJi-Sook Han

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adenoma, Islet Cell

Islet Cell Adenoma arises in the islet cells, which are insulin producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on Islet Cell Adenoma here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.