PMID: 9420256Jan 7, 1998Paper

71-kilodalton heat shock cognate protein acts as a cellular receptor for syncytium formation induced by human T-cell lymphotropic virus type 1

Journal of Virology
Y SagaraY Maeda

Abstract

We previously reported that the region corresponding to amino acids 197 to 216 of the gp46 surface glycoprotein (gp46-197) served as a binding domain for the interaction between gp46 and trypsin-sensitive membrane components of the target cell, leading to syncytium formation induced by human T-cell lymphotropic virus type 1 (HTLV-1)-bearing cells. Our new evidence shows that the 71-kDa heat shock cognate protein (HSC70) acts as a cellular receptor for syncytium formation. Using affinity chromatography with the peptide gp46-197, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, we isolated three components (bands A, B, and C) from MOLT-4 cell lysate which exhibited specific interactions with gp46 and inhibitory activities for syncytium formation induced by HTLV-1-bearing cells. Band A and B components were identified as HSC70 and beta-actin, respectively, through amino acid sequencing by tandem mass spectrometry and immunostaining with specific monoclonal antibodies. Band C is likely to be a nonprotein component, because full activity for syncytium formation was seen after extensive trypsin digestion. Anti-HSC70 monoclonal antibody clearly blocked syncytium formation in a coculture of HTLV-1-bearing cells an...Continue Reading

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Citations

Nov 24, 1999·The Journal of General Virology·M A Sommerfelt
Sep 13, 2005·Oncogene·Nicolas ManelMarc Sitbon
Jul 1, 2006·Journal of Virology·David GhezOlivier Hermine
Mar 22, 2002·Journal of Virology·Carlos A GuerreroCarlos F Arias
Feb 21, 2002·Cancer Investigation·Bernard J Poiesz
May 15, 2003·Cancer Investigation·Bernard J PoieszDavid Choi
Mar 24, 2016·Viruses·Christine Gross, Andrea K Thoma-Kress
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May 23, 2014·Journal of Biosciences·Mandar S Paingankar, Vidya A Arankalle
Feb 15, 2001·AIDS Research and Human Retroviruses·Y SagaraY Maeda
Nov 20, 2012·Virology Journal·Zhongqi BianZhaoxin Zheng
Dec 9, 2009·Expert Review of Anti-infective Therapy·Péter BagossiJózsef Tözsér
Oct 15, 2013·Autophagy·François StricherSylviane Muller
May 16, 2014·BioMed Research International·Nayeli Alva-MurilloAlejandra Ochoa-Zarzosa
Jul 3, 2009·Biological & Pharmaceutical Bulletin·Hiromi NabeshiYasuo Tsutsumi
Dec 1, 2010·Retrovirology·David GhezOlivier Hermine
Sep 27, 2018·Scientific Reports·Juliana Echevarria-LimaRussolina B Zingali
Aug 9, 2020·Frontiers in Microbiology·Pengpeng ZhuJiyong Zhou
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Jan 10, 2001·International Journal of Cancer. Journal International Du Cancer·T YoshidaN Yamamoto
Feb 23, 2000·Virology·S R Trejo, L Ratner

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