7,12-dimethylbenz(a)anthracene treatment of a c-rel mouse mammary tumor cell line induces epithelial to mesenchymal transition via activation of nuclear factor-kappaB

Cancer Research
Sangmin Ryan ShinGail E Sonenshein

Abstract

The aberrant expression of the nuclear factor-kappaB (NF-kappaB) c-Rel subunit that occurs in many human breast cancers can play a causal role in tumorigenesis as judged by findings with a mouse mammary tumor virus (MMTV)-c-rel transgenic mouse model, in which 31.6% of mice developed one or more mammary tumors after a long latency. Interestingly, none of the cell lines established from the mammary tumors grew in soft agar. To begin to test the hypothesis that a prototypic carcinogen insult can promote a more invasive, mesenchymal phenotype, a cell line established from a MMTV-c-rel mammary tumor rel-3983 was treated in culture with the polycyclic aromatic hydrocarbon 7,12-dimethylbenz(a)anthracene (DMBA; rel-3983D cells) or DMSO vehicle (rel-3983V cells). Rel-3983D cells displayed an increased rate of proliferation, displayed growth to a higher cell density, and acquired the ability to grow in soft agar and in Matrigel compared with the parental rel-3983 or vehicle-treated rel-3983V cells. Consistent with a more mesenchymal phenotype, rel-3983D cells showed loss of E-cadherin expression as judged by immunofluorescence microscopy. Compared with control cells, rel-3983D displayed increased NF-kappaB binding and higher levels of t...Continue Reading

References

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Citations

Mar 21, 2007·Nature Cell Biology·Xiaobo WangGail E Sonenshein
Sep 16, 2006·Biological Chemistry·Jennifer J SchlezingerDavid H Sherr
Jan 30, 2016·Nature Reviews. Drug Discovery·Fabrizio MarcucciRuggero De Maria
Dec 17, 2015·ACS Nano·Manish K JaiswalAkhilesh K Gaharwar
Jul 8, 2009·Expert Review of Molecular Diagnostics·Pankaj TanejaKazushi Inoue
May 8, 2018·International Journal of Molecular Sciences·Supraja NarasimhanDavid H Sherr
Jun 13, 2018·Journal of Cancer Research and Therapeutics·Mónica Sierra-MartinezOctavio Daniel Reyes-Hernández
May 20, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·David H Sherr
Feb 7, 2008·Journal of Cellular Biochemistry·Chengyin MinGail E Sonenshein
Jan 7, 2010·Journal of Cellular Biochemistry·Junfeng WuZhi-Xiong Jim Xiao
Feb 11, 2010·Genesis : the Journal of Genetics and Development·Nicolas CurrierIsabel Dominguez
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suzanne D ClutterRaphael Hirsch
Nov 19, 2020·Environmental Health : a Global Access Science Source·Meriem KoualXavier Coumoul
Oct 12, 2010·Biochemical and Biophysical Research Communications·Libing SongJun Li

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