PMID: 9528917Apr 7, 1998Paper

72-kDa heat shock protein and mRNA expression after controlled cortical impact injury with hypoxemia in rats

Journal of Neurotrauma
M ChenS H Graham

Abstract

As part of the stress response, the 72 kDa heat shock protein (hsp72) is induced in neurons after ischemic and traumatic brain injury (TBI). To examine the stress response after TBI with secondary insult, we examined the regional and cellular expression of hsp72 mRNA and protein after controlled cortical impact (CCI) injury with secondary hypoxemia and mild hypotension in rats. Rats were killed at 6, 8, 24, 72, or 168 h after trauma. Naive and sham-operated rats were used as controls. Brains were removed, and in situ hybridization (n = 2/group), immunocytochemistry (n = 4/group), and Western blot analysis (n = 3 to 5/group) for hsp72 was performed. Hsp72 mRNA was expressed in neurons in the ipsilateral cortex, CA3 region of the hippocampus, hilus, and dentate gyrus at 6 h. Hsp72 mRNA was expressed primarily in the ipsilateral cortex, at 24 h, and by 72 h hsp72 mRNA expression returned to near basal levels. Hsp72 protein was seen in ipsilateral cortical neurons, hilar neurons, and neurons in the medial aspect of the CA3 region of the hippocampus (CA3-c) at 24 h. At 72 h, hsp72 immunoreactivity was reduced versus 24 h in these same regions, but it was increased versus baseline. Western blot analysis confirmed an increase in hsp72...Continue Reading

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Citations

Apr 25, 2012·Journal of Molecular Histology·Ting XuXingxing Gu
May 14, 2003·Neurobiology of Disease·Hilaire J ThompsonTracy K McIntosh
May 2, 2008·Journal of Neurotrauma·Douglas S DeWitt, Donald S Prough
Aug 12, 2010·Journal of Neurotrauma·Andrew K OttensJitendra R Dave
Jan 4, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Ibolja Cernak
Aug 6, 2010·Progress in Neurobiology·R Anne StetlerJun Chen
Mar 26, 2010·Journal of Neuroscience Research·Paula van TijnDavid F Fischer
Sep 1, 2006·Developmental Neuroscience·J'mir L CousarRobert S B Clark
Aug 15, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Zaorui ZhaoBogdan A Stoica
Jan 26, 2000·Journal of Neurochemistry·R S ClarkS H Graham
Dec 28, 2002·Journal of Neuroscience Research·Vemuganti L Raghavendra RaoRobert J Dempsey
Feb 17, 2000·Current Opinion in Neurology·H L Laurer, T K McIntosh
Sep 22, 2019·Neuroscience Letters·Leonardo Pignataro
Jul 25, 2021·Trends in Neurosciences·Janani Saikumar, Nancy M Bonini

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