8-Azido-nucleotides as substrates of Torpedo electric organ apyrase. effect of photoactivation on apyrase activity

Brain Research Bulletin
E MartíC Solsona

Abstract

Ecto-apyrase is a widespread enzymatic activity that hydrolyses tri- and diphosphonucleotides and consequently controls the amount of available extracellular ATP and ADP. In the nervous system, purines have important neuromodulatory actions, acting at pre- and postsynaptic sites, and consequently, ecto-apyrase may play an indirect role in the modulation of nucleotide- and nucleoside-mediated processes. The azido-nucleotides have been largely employed to characterize the nucleotide binding sites of several proteins. In the present work the azido-nucleotides are described as putative substrates for apyrase activity in a presynaptic plasma membrane preparation (PSPM) from the Torpedo electric organ. Both 8-N3-ATP and 8-N3-ADP were hydrolyzed in a calcium-dependent manner showing Vmax of 23.8 +/- 4.8 and 14.5 +/- 3 U/mg of protein, and Km values (in microM) of 116 +/- 39 and 119 +/- 4, respectively. Vmax for calcium-dependent hydrolysis of ATP and ADP were significantly higher: 59.2 +/- 3.9 and 32.9 +/- 3.5 U/mg of protein respectively, while Km values did not show any significant differences regarding azido-nucleotides: 83.8 +/- 12 microM for Ca2+-ATP and 121 +/- 34 microM for Ca2+-ADP. The photoactivation of the PSPM in the prese...Continue Reading

References

Nov 15, 1979·Analytical Biochemistry·P A LanzettaO A Candia
May 2, 1991·European Journal of Pharmacology·R A Cunha, A M Sebastião
Sep 1, 1990·Cellular and Molecular Neurobiology·V A EterovićM G McNamee
Feb 1, 1987·Journal of Neurochemistry·P J Richardson, S J Brown
Oct 1, 1989·Journal of Neurochemistry·A K NagyA V Delgado-Escueta
May 1, 1981·Journal of Neurochemistry·N Morel, F M Meunier
Apr 1, 1980·Journal of Neurochemistry·M IsraëlP Frachon
Oct 1, 1994·Trends in Neurosciences·H Zimmermann
Nov 1, 1993·Archives of Biochemistry and Biophysics·F Rodríguez-PascualM T Miras-Portugal
Jan 1, 1993·Journal of Neurochemistry·S James, P J Richardson
Jul 1, 1996·British Journal of Pharmacology·E MartíC Solsona
May 3, 2002·European Journal of Biochemistry·Aileen F KnowlesMae Wu-Weis

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Citations

Apr 6, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M R SchetingerD Balz
Jun 21, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·A C da SilvaM R C Schetinger

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