8-Cl-cAMP and its metabolite, 8-Cl-adenosine induce growth inhibition in mouse fibroblast DT cells through the same pathways: protein kinase C activation and cyclin B down-regulation

Journal of Cellular Physiology
Young-Ho AhnSeung Hwan Hong

Abstract

8-Chloro-cyclic AMP (8-Cl-cAMP) is known to be most effective in inducing growth inhibition and differentiation of a number of cancer cells. Also, its cellular metabolite, 8-Cl-adenosine was shown to induce growth inhibition in a variety of cell lines. However, the signaling mechanism that governs the effects of 8-Cl-cAMP and/or 8-Cl-adenosine is still uncertain and it is not even sure which of the two is the key molecule that induces growth inhibition. In this study using mouse fibroblast DT cells, it was found that adenosine kinase inhibitor and adenosine deaminase could reverse cellular growth inhibition induced by 8-Cl-cAMP and 8-Cl-adenosine. And 8-Cl-cAMP could not induce growth inhibition in the presence of phosphodiesterase (PDE) inhibitor, but 8-Cl-adenosine could. We also found that protein kinase C (PKC) inhibitor could restore this growth inhibition, and both the 8-Cl-cAMP and 8-Cl-adenosine could activate the enzymatic activity of PKC. Besides, after 8-Cl-cAMP and 8-Cl-adenosine treatment, cyclin B was down-regulated and a CDK inhibitor, p27 was up-regulated in a time-dependent manner. These results suggest that it is not 8-Cl-cAMP but 8-Cl-adenosine which induces growth inhibition, and 8-Cl-cAMP must be metabolize...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·E G Krebs, J A Beavo
Jul 1, 1992·Cancer Causes & Control : CCC·E V BanderaG Wilkinson
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B R SevetsonJ C Lawrence
Oct 1, 1994·Journal of Pharmaceutical and Biomedical Analysis·J CummingsW R Miller
Feb 1, 1994·Trends in Biochemical Sciences·L V Dekker, P J Parker
Mar 1, 1996·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J CummingsW R Miller
Jul 3, 1998·International Journal of Cancer. Journal International Du Cancer·M YoshidaN Saijo
Nov 14, 1998·The American Journal of Physiology·C M Liedtke, T S Cole
Jun 30, 2000·Biochemical and Biophysical Research Communications·S N KimS H Hong
Jun 8, 2001·International Journal of Cancer. Journal International Du Cancer·S N KimS H Hong
Mar 12, 2002·The Journal of Investigative Dermatology·D T DransfieldW B Bollag
Jun 21, 2002·Trends in Cell Biology·Philip J S Stork, John M Schmitt
Nov 27, 2002·Cancer Investigation·Olivera GrbovicSelma Kanazir

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Citations

Jun 6, 2015·Biochemical and Biophysical Research Communications·Ki Young ChoiSeung Hwan Hong
Sep 29, 2012·Journal of Cellular Physiology·Ki Young ChoiSeung Hwan Hong
Sep 15, 2006·Journal of Cellular Physiology·Young-Ho AhnSeung Hwan Hong

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