8-hydroxy-2'-deoxyguanosine in leukocyte DNA of spermatic vein as a biomarker of oxidative stress in patients with varicocele

The Journal of Urology
Shiou-Sheng ChenYau-Huei Wei

Abstract

We examined oxidative damage to leukocyte DNA in the spermatic vein and sperm DNA of patients with varicocele. A total of 32 young male patients with varicocele (group 1), 20 young male patients with subclinical varicocele (group 2) and 15 normal young males without varicocele (group 3) were recruited in this study. Varicocele and subclinical varicocele were confirmed by physical examination and Doppler ultrasonography. Blood samples were drawn from peripheral and spermatic veins of controls and patients before varicocelectomy. The 8-hydroxy-2'-deoxyguanosine (8-OHdG) in leukocyte DNA and sperm DNA were measured by high performance liquid chromatography. The 4977 bp deletion of mitochondrial DNA (mtDNA) in spermatozoa was detected by polymerase chain reaction. The mean 8-OHdG level +/- SD in leukocyte DNA of spermatic veins was significantly higher than that of corresponding peripheral veins in groups 1 and 2 (12.39 +/- 2.90 vs 7.11 +/- 0.75/10 deoxyguanosine for group 1 and 10.28 +/- 2.43 vs 6.82 +/- 0.62/10 deoxyguanosine for group 2, p <0.001). The 8-OHdG level in leukocyte DNA of the spermatic vein and 8-OHdG in sperm DNA were highest in group 1 followed by those in groups 2 and 3, and correlated inversely with motility, mo...Continue Reading

References

Apr 1, 1991·The Journal of Urology·R D McClureH Hricak
Jan 1, 1994·Human Reproduction·J W Marsman, R Schats
Apr 13, 1996·Mutation Research·C G FragaB N Ames
May 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·E W AustinR J Bull
Jul 27, 1999·World Journal of Urology·H D Nöske, W Weidner
May 23, 2001·Free Radical Biology & Medicine·S S ChenY H Wei

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Citations

Sep 13, 2008·Journal of Endocrinological Investigation·C FiniF Tiziani
Aug 7, 2014·TheScientificWorldJournal·Akira KomiyaHideki Fuse
Jul 20, 2012·Reproductive Biomedicine Online·Ying-Jun WangWei-Li Zhang
Nov 1, 2011·Fertility and Sterility·Armand Zini, Gert Dohle
Jan 27, 2009·Urology·Ashok AgarwalEdmund Sabanegh
Dec 18, 2007·The Journal of Urology·Shiou-Sheng ChenYau-Huei Wei
Dec 18, 2007·Fertility and Sterility·Fotini PapachristouTheodore Lialiaris
Dec 18, 2007·The Journal of Urology·Raimondo Maximilian CervellioneAdrian Bianchi
Aug 3, 2007·Fertility and Sterility·Shu-Huei KaoYau-Huei Wei
Jun 15, 2007·Fertility and Sterility·Philip WerthmanDonald P Evenson
Feb 21, 2013·Andrologia·N G GashtiS T Dalivandan
Nov 6, 2007·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Marcello CocuzzaAshok Agarwal
Jun 12, 2018·Journal of Pineal Research·Annia Galano, Russel J Reiter
May 12, 2010·Journal of the Chinese Medical Association : JCMA·Shiou-Sheng Chen, William J Huang
Feb 19, 2008·Human Reproduction Update·Kelton Tremellen
Jun 19, 2008·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Fnu DeepinderAshok Agarwal
Jul 20, 2013·Reproductive Medicine and Biology·Akira KomiyaHideki Fuse
Sep 1, 2016·Porto Biomedical Journal·Daniel Filipe Cruz, Margarida Fardilha

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