99mTc-Evans blue dye for mapping contiguous lymph node sequences and discriminating the sentinel lymph node in an ovine model

Annals of Surgical Oncology
Chris TsopelasBarry E Chatterton

Abstract

The aim of this study was to investigate the potential of (99m)Tc-Evans blue for discriminating the sentinel lymph node in multitiered lymph node sequences by using an ovine model. (99m)Tc-Evans blue is an agent that has both radioactive and color signals in a single dose. Previous studies in smaller animal models suggested that this agent could have advantages over the dual-injection technique of radiocolloid/blue dye. Doses of (99m)Tc-Evans blue ( approximately 21 MBq) containing Evans blue dye (approximately 4 mg) were administered to the hind limbs or fore limbs of sheep to map the lymphatic drainage patterns, validate its ability to identify the sentinel lymph node, and examine the reproducibility of the technique. The study protocol was repeated with (99m)Tc-antimony trisulfide colloid and Patent Blue V dye. After the operative exposure, lymph nodes were identified with the gamma probe and then excised and analyzed for radioactivity (percentage of injected dose) and blue color. After the administration of (99m)Tc-Evans blue, all lymph nodes harvested (35 of 35) in either short chains or long basins were hot and blue. The sentinel lymph nodes concentrated more radioactivity than the second-tier nodes to the extent of 2:1 t...Continue Reading

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Citations

Nov 29, 2008·Annals of Nuclear Medicine·Chris TsopelasBarry E Chatterton
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yu WangXiaoyuan Chen
Jan 5, 2011·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·V WhiteM Carr
Sep 2, 2009·Nuclear Medicine and Biology·Eutimio Gustavo Fernández NúñezRoberto Pasqualini
Mar 26, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiancheng ChenXia Zhao
Jun 1, 2018·Contrast Media & Molecular Imaging·Linpeng YaoFeng Chen
May 31, 2018·Journal of Materials Science. Materials in Medicine·Aidan CousinsBenjamin Thierry
Aug 11, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohammad AbdallahNatalie L Trevaskis

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