99mTc-labeled tricarbonyl his-CNA35 as an imaging agent for the detection of tumor vasculature

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Gilles MeesChristophe Van de Wiele

Abstract

Given the importance of angiogenesis for a tumor's survival and growth, several therapeutic strategies rely on the selective inhibition of angiogenesis and the destruction of existing tumor vasculature. These strategies raise the need for a noninvasive tool to evaluate tumor vasculature. We describe the radiosynthesis and evaluation of an imaging tracer that specifically binds tumor subendothelial collagen and thereby images tumor vasculature. (99m)Tc-tricarbonyl was prepared and labeled with His-collagen-binding adhesion protein 35 (CNA35). After in vitro specificity testing, in vivo biodistribution and dosimetric studies were performed in healthy nude mice via planar imaging. (99m)Tc-(CO)(3) His-CNA35 was evaluated for in vivo imaging of tumor vasculature in a HT29 colorectal carcinoma xenograft. The labeling procedure yielded a compound with 95%-99% radiochemical purity and good in vitro stability. An in vitro binding test confirmed specificity and functionality. (99m)Tc-(CO)(3) His-CNA35 rapidly cleared from the blood and predominantly accumulated in the kidneys and liver. The effective dose for a proposed single injection of 500 MBq of (99m)Tc-(CO)(3) His-CNA35 is 3.70 mSv per organ or 2.01 mSv/g of tissue. Tumors were suc...Continue Reading

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Citations

Dec 10, 2014·PloS One·Stijn J A AperMaarten Merkx
Sep 25, 2014·Molecular Imaging·Sanne de JongMarc A M J van Zandvoort
Mar 1, 2014·Circulation Research·Hans J de HaasJagat Narula
Sep 12, 2013·Biomedizinische Technik. Biomedical Engineering·Julia FreseFabian Kiessling
Feb 13, 2021·Journal of Personalized Medicine·Nithya Subrahmanyam, Hamidreza Ghandehari
Jul 28, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Sina TavakoliReto Asmis

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