A β-galactosidase-expressing E. coli culture as an alternative test to identify skin sensitizers and non-sensitizers

Journal of Toxicology and Environmental Health. Part a
Mahesh Raj NepalTae Cheon Jeong

Abstract

Although the Organization for Economic Cooperation and Development (OECD) has adopted several in vitro methods with reasonable predictive capacity, alternative methods for identifying skin sensitizers and non-sensitizers with reliability and simplicity are still required for more efficient and economic prediction. The present study was to design an in vitro system with the use of a β-galactosidase-expressing E. coli culture for simpler but sufficiently accurate classification of skin sensitizers and non-sensitizers. A LacZ gene-containing E. coli strain that is capable of producing β-galactosidase enzyme was induced by isopropyl β-D-1-thiogalactopyranoside with concomitant treatment with test chemicals. After 6-hr incubation, cells were lysed and β-galactosidase enzyme activity was monitored colorimetrically by using O-nitrophenyl-D-galactopyranoside as a substrate. Following optimization of several experimental conditions, 22 skin sensitizers and 11 non-sensitizers were examined to assess predictive capacity of this method. The results indicated that predictivity was as follows: 90.9% sensitivity, 81.8% specificity, and 87.9% accuracy, when 17.3% of control activity was used as the cut-off value to separate sensitizers from no...Continue Reading

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Citations

Jul 19, 2018·Journal of Toxicology and Environmental Health. Part a·Min Kook KimByung-Mu Lee
Sep 12, 2019·Journal of Toxicology and Environmental Health. Part a·Mahesh Raj NepalTae Cheon Jeong
Apr 10, 2021·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Kyu-Bong KimByung-Mu Lee
Jul 2, 2021·Journal of Toxicology and Environmental Health. Part a·Geon Ho KimTae Cheon Jeong

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