A 10-Gene Classifier for Indeterminate Thyroid Nodules: Development and Multicenter Accuracy Study

Thyroid : Official Journal of the American Thyroid Association
Hernán E GonzálezSoledad Urra

Abstract

In most of the world, diagnostic surgery remains the most frequent approach for indeterminate thyroid cytology. Although several molecular tests are available for testing in centralized commercial laboratories in the United States, there are no available kits for local laboratory testing. The aim of this study was to develop a prototype in vitro diagnostic (IVD) gene classifier for the further characterization of nodules with an indeterminate thyroid cytology. In a first stage, the expression of 18 genes was determined by quantitative polymerase chain reaction (qPCR) in a broad histopathological spectrum of 114 fresh-tissue biopsies. Expression data were used to train several classifiers by supervised machine learning approaches. Classifiers were tested in an independent set of 139 samples. In a second stage, the best classifier was chosen as a model to develop a multiplexed-qPCR IVD prototype assay, which was tested in a prospective multicenter cohort of fine-needle aspiration biopsies. In tissue biopsies, the best classifier, using only 10 genes, reached an optimal and consistent performance in the ninefold cross-validated testing set (sensitivity 93% and specificity 81%). In the multicenter cohort of fine-needle aspiration b...Continue Reading

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Citations

Dec 20, 2017·Endocrine-related Cancer·Sergio Vargas-SalasHernán E González
Jun 6, 2020·Diagnostic Cytopathology·Neeta KumarSanjay Gupta
Jun 4, 2020·The Journal of Clinical Endocrinology and Metabolism·Giorgio GraniCosimo Durante
Jan 10, 2019·Archives of Endocrinology and Metabolism·Carolina Ferraz
Aug 29, 2018·International Journal of Molecular Sciences·Po-Sheng YangShih-Ping Cheng
Jan 8, 2020·Thyroid : Official Journal of the American Thyroid Association·Mark ZafereoHernán E González
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Lamya RezigStefano Caldarelli
Jan 26, 2021·International Journal of Endocrinology·Zexin LiWencan Xu
Feb 13, 2021·International Journal of Molecular Sciences·Stanislaw SupplittIzabela Laczmanska
Mar 6, 2021·Current Opinion in Otolaryngology & Head and Neck Surgery·Giorgio GraniCosimo Durante

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Methods Mentioned

BETA
biopsies
Assay
biopsy

Software Mentioned

BLAST
Endopredict
GraphPad
NLDA
Primer
RosettaGX
ThyroSeq
SPSS

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