A 100 amino acid region in the GABA rho 1 subunit confers robust homo-oligomeric expression

Neuroreport
A S HackamG R Cutting

Abstract

Retinal gamma-aminobutyric acid type C (GABAC) receptors are believed to be composed of rho subunits. Although rho 1 and rho 2 are over 80% similar, the whole-cell currents generated by rho 1 receptors in Xenopus oocytes are significantly greater than those generated by rho 2 receptors. In this study, chimeric subunits containing different portions of human rho 1 and human rho 2 were created to localize sequences facilitating robust rho 1 expression. Our results indicate that these sequences reside in a 100 amino acid domain in the N-terminus of rho 1, and may involve N-linked glycosylation. Since the N-terminus also contains subunit assembly signals, rho 1 receptors may be formed more efficiently than rho 2 receptors. Therefore, this study furthers our understanding of the molecular basis of GABA-mediated inhibition in the retina.

References

Jan 1, 1991·Progress in Neurobiology·L Sivilotti, A Nistri
Dec 1, 1990·The Journal of Cell Biology·P Blount, J P Merlie
Jan 16, 1987·Cell·G Brawerman
Jun 1, 1987·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J Pelletier, N Sonenberg
Jul 1, 1993·Trends in Pharmacological Sciences·G Bonanno, M Raiteri
Jan 14, 1993·Nature·H Qian, J E Dowling
Dec 1, 1995·Trends in Neurosciences·J Bormann, A Feigenspan
Aug 1, 1996·Current Opinion in Biotechnology·D F Wyss, G Wagner
Oct 1, 1995·Journal of Neurophysiology·J Zhang, M M Slaughter

❮ Previous
Next ❯

Citations

Mar 10, 2001·Trends in Pharmacological Sciences·D ZhangS A Lipton
Nov 24, 1999·Clinical and Experimental Pharmacology & Physiology·M Chebib, G A Johnston
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Martinez-Torres, R Miledi
Oct 11, 2001·Biological Chemistry·R Enz
Jul 17, 1998·Vision Research·R Enz, G R Cutting
Mar 14, 2002·Journal of Neuroscience Research·Frédéric DidelonEnrico Cherubini
Sep 19, 2001·The Journal of Biological Chemistry·C BüttnerG Schmalzing
Jun 18, 2002·The Journal of Biological Chemistry·Isabella SartoWerner Sieghart
Apr 26, 2000·Journal of Medicinal Chemistry·M Chebib, G A Johnston

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.