A 100 bp GAGA motif-containing sequence in AGAMOUS second intron is able to suppress the activity of CaMV35S enhancer in vegetative tissues

PloS One
Ruochen LiuYan Pei

Abstract

Flower-specific promoters enable genetic manipulation of floral organs to improve crop yield and quality without affecting vegetative growth. However, the identification of strong tissue-specific promoters is a challenge. In addition, information on cis elements that is able to repress gene expression in vegetative tissues remains limited. Here, we report that fusing a 35S enhancer to the stamen- and carpel-specific NtAGIP1 promoter derived from the tobacco AGAMOUS second intron (AGI) can significantly increase the promoter activity. Interestingly, although the activity of the new promoter extends to sepals and pedicles, it does not cross the boundary of the reproductive organs. Serial deletion of the AGI and chromatin immunoprecipitation (ChIP) assay reveal a 100-bp fragment that contains a conserved GAGA factor binding motif contributes to the flower specificity by mediating histone H3 lysine 27 trimethylation (H3K27me3) modification of the promoter. Furthermore, this fragment shows significant suppressive effect on the activity of the 35S enhancer in vegetative tissues, consequently, resulting in a significant increase of the activity of 35S enhancer:AGI chimeric promoter without sacrifice of its specificity in inflorescence.

References

Jul 27, 1999·Science·M A BuschD Weigel
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·J Conner, Z Liu
Mar 29, 2005·Nature Biotechnology·Jacqueline A PaineRachel Drake
Sep 3, 2010·The Plant Journal : for Cell and Molecular Biology·Jonathan M PlettSharon Regan
Mar 2, 2011·Nature Reviews. Genetics·Chin-Tong Ong, Victor G Corces
Feb 20, 2013·Advances in Genetics·Judith A Kassis, J Lesley Brown
Jan 27, 2015·Annual Review of Plant Biology·Iva Mozgova, Lars Hennig
Apr 11, 2015·Trends in Genetics : TIG·Li Pu, Zinmay Renee Sung
Nov 3, 2015·Current Opinion in Biotechnology·Wusheng Liu, C Neal Stewart
Sep 22, 2019·International Journal of Molecular Sciences·Haiqiang DongWei Li

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Methods Mentioned

BETA
transgenic
PCR
immunoprecipitation
ChIP

Software Mentioned

Image Pro - Plus

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