PMID: 3768331Sep 9, 1986Paper

A 10S particle released from deoxyribonuclease-sensitive regions of HeLa cell nuclei contains the 86-kilodalton-70-kilodalton protein complex

Biochemistry
M Yaneva, H Busch

Abstract

Digestion of HeLa cell nuclei with micrococcal nuclease or deoxyribonuclease I (DNase I) released the 86-kilodalton-70-kilodalton (kDa) protein complex in particles sedimenting at approximately 10 S in sucrose density gradients. Immunoaffinity-purified 32P-labeled complexes contained 86- and 70-kDa polypeptides with phosphorylated serine residues and DNA fragments, of which the largest was 110 base pairs long. Digestion of nick-translated nuclei with micrococcal nuclease released 32P-labeled 10S particles that were immunoaffinity-purified; they contained labeled 110-base-pair DNA fragments. The micrococcal nuclease digests were analyzed by two-dimensional electrophoresis, which separated nucleosomes in the first dimension and the associated proteins in the second. Western blots of the separated proteins showed that the 86-kDa-70-kDa complex was associated with the mono-, di-, and trinucleosomes. A more extensive electrophoretic separation revealed that the 10S particle from nick-translated nuclei migrated with a subfraction of the mononucleosomes that lacked H1 histones. These results suggest that the 10S particle which contains the 86-kDa-70-kDa complex is associated with an unfolded nucleosome that is present in DNase I sensi...Continue Reading

References

Apr 1, 1979·Developmental Biology·A LevittH Cedar
Sep 3, 1976·Science·H Weintraub, M Groudine
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Lacy, R Axel
Jul 26, 1985·Biochimica Et Biophysica Acta·M YanevaH Busch
Jan 1, 1984·Methods in Enzymology·H Busch
Dec 1, 1980·Experimental Cell Research·K AnderssonB Daneholt
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
May 27, 1982·Nature·S Weisbrod
Jan 1, 1980·Cell·S WeisbrodH Weintraub
Mar 29, 1983·Biochemical and Biophysical Research Communications·E DurbanH Busch
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B GazitH Cedar
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L Levinger, A Varshavsky
Jan 1, 1980·Annual Review of Biochemistry·J D McGhee, G Felsenfeld
Feb 1, 1981·Scientific American·R D Kornberg, A Klug

❮ Previous
Next ❯

Citations

Jun 1, 1992·Molecular Biology Reports·C H ChouW H Reeves
Oct 1, 1990·Molecular Immunology·J Wen, M Yaneva
Oct 8, 1991·Biochimica Et Biophysica Acta·M Yaneva, S Jhiang
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·N ShakibaiS Eisenberg
Oct 1, 1990·The Journal of Experimental Medicine·M H StuiverP C van der Vliet
Apr 16, 2002·The EMBO Journal·Les A Hanakahi, Stephen C West
Oct 25, 1991·Nucleic Acids Research·S Paillard, F Strauss
Sep 25, 1994·Nucleic Acids Research·M OnoJ D Capra
Oct 1, 1990·The Journal of Clinical Investigation·B S PrabhakarA L Notkins
Mar 16, 2016·Experimental Cell Research·Katsuya Iuchi, Tatsuo Yagura
Apr 1, 1992·Experimental Cell Research·L L Li, N H Yeh
Jan 31, 1991·Biochemical and Biophysical Research Communications·W W ZhangH Busch
Jul 15, 1992·Biochemical and Biophysical Research Communications·W W Zhang, M Yaneva
Feb 1, 1996·Rheumatic Diseases Clinics of North America·C GalperinM E Gershwin
May 29, 2001·The Journal of Biological Chemistry·P DouglasS P Lees-Miller
Jan 1, 1992·Autoimmunity·R G DalzielJ P Quinn
Mar 14, 2001·American Journal of Physiology. Cell Physiology·E M LynchD V Faller
Jan 26, 2005·The Journal of Biological Chemistry·Jia Li, David F Stern
Mar 1, 1997·Molecular and Cellular Biology·B K SingletonP A Jeggo
Jun 15, 1992·Biochimica Et Biophysica Acta·J P QuinnA R Farina
Aug 1, 1992·Clinical Immunology and Immunopathology·L Abu-Elheiga, M Yaneva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.