A 178-kb BAC transgene imprints the mouse Gtl2 gene and localizes tissue-specific regulatory elements

Genomics
Aleksey YevtodiyenkoJennifer V Schmidt

Abstract

The regulation of genomic imprinting, the allele-specific expression of an autosomal gene, is complex and poorly understood. Imprinted genes are organized in clusters, where cis-acting regulatory elements are believed to interact to control multiple genes. We have used BAC transgenesis in the mouse to begin to delineate the region of DNA required for proper expression and imprinting of the mouse Delta-like1 (Dlk1) and Gene-trap locus2 (Gtl2) imprinted genes. We demonstrate that the Gtl2 gene is expressed from a BAC transgene in mouse embryo and placenta only upon maternal inheritance, as is the endogenous Gtl2 gene. Gtl2 is therefore properly imprinted on the BAC in an ectopic chromosomal location and must carry with it all necessary imprinting regulatory elements. Furthermore, we show that the BAC Gtl2 gene is expressed at levels approaching those of the endogenous gene only in the brain of adult animals, not in other sites of endogenous expression such as the pituitary, adrenal, and skeletal muscle. These data localize the enhancer(s) for brain Gtl2 expression, but not those for other tissues, to the DNA contained within the BAC clone. As the Dlk1 gene is not expressed from the BAC in any tissues, it must require additional e...Continue Reading

Citations

Oct 4, 2006·BMC Genetics·Ekaterina Y SteshinaJennifer V Schmidt
Oct 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nan Li, Laura Carrel
Jan 5, 2011·Journal of Biomedicine & Biotechnology·S J TunsterR M John
May 19, 2012·PloS One·Eric D RogersJennifer V Schmidt
Apr 30, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Sylvie CroteauAnna K Naumova
Aug 17, 2010·Yi chuan = Hereditas·Li-Xia ZhaoHuan-Min Zhou
Aug 3, 2010·Briefings in Functional Genomics·Rosalind M John
May 13, 2008·Trends in Genetics : TIG·Simao Teixeira da RochaAnne C Ferguson-Smith
Sep 26, 2012·Annual Review of Genomics and Human Genetics·Mary A M CleatonAnne C Ferguson-Smith

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