A 2-D liquid-phase chromatography for proteomic analysis in plant tissues

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Andrea PirondiniNelson Marmiroli

Abstract

Two-dimensional liquid chromatography based on a high-performance chromatofocusing in the first dimension followed by high-resolution reversed-phase chromatography in the second dimension can be used as a complementary approach to protein separation with two-dimensional gel electrophoresis. In this work, Arabidopsis thaliana proteins obtained from different tissue extracts were resolved by using a new automated system, ProteomeLab PF 2D commercialized by Beckman Coulter (Fullerton, CA, USA). In particular, protein patterns obtained after two different extraction procedures (MgSO4 and urea buffer) were compared. Reproducibility of the protein patterns was also confirmed in different injections of the same sample and in the comparative analyses of some proteins by MALDI-TOF/MS. Computer analysis of the chromatograms revealed that with this two-dimensional liquid phase technique, hundreds of "virtual bands" can be identified and compared in crude plant protein lysates.

Citations

Dec 21, 2010·International Journal of Phytoremediation·Giovanna VisioliNelson Marmiroli
Oct 21, 2011·Journal of Biomedicine & Biotechnology·Mina KimYang-Hoon Kim
Dec 2, 2011·Journal of Biomedicine & Biotechnology·Tamara ZimaroJorgelina Ottado
Sep 12, 2009·Journal of Separation Science·Oscar AguilarMarco Rito-Palomares
Jul 27, 2007·Proteomics·Jesús V JorrínMa Angeles Castillejo
Jun 3, 2008·Environmental Pollution·C RustichelliN Marmiroli
Dec 27, 2012·Journal of Proteomics·Giovanna Visioli, Nelson Marmiroli
Jul 1, 2010·Journal of Biomedicine & Biotechnology·Raquel González-FernándezJesús V Jorrín-Novo
Sep 8, 2012·Journal of Proteome Research·Mariasole Di CarliMarcello Donini

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