A 22-amino-acid peptide regulates tight junctions through occludin and cell apoptosis

PeerJ
Maoying ZhuDeyu Chen

Abstract

Occludin is a structural protein of tight junctions (TJ) in the blood-testis barrier (BTB). A 22-amino-acid peptide (22AA) in the second extracellular loop can reversibly regulate TJ, but its regulatory mechanism is unknown. In this study, a 22AA-induced TJ destruction animal model was constructed to investigate the effect of 22AA on Sertoli cells (SCs) and spermatid counts and cell apoptosis at different time points using a multiplex immunofluorescence technique. The effect of 22AA on the location and distribution of occludin was analyzed via dual confocal fluorescence microscope. Western blotting was used to analyze dynamic changes in occludin expression. Real-time RT-PCR was used to analyze miR-122-5p expression changes. Sperm density counts and mating methods were used to analyze the effect of 22AA on fertility in mice. The results showed that 22AA promoted SC and spermatid apoptosis, downregulated occludin, upregulated miR-122-5p, and decreased sperm density and litter size before 27 days (27D). After 27D, the expression of occludin increased again, miR-122-5p expression decreased again, both sperm density and litter size returned to normal, apoptosis stopped, and spermatogenesis began to recover. Therefore, it can be conc...Continue Reading

References

Jun 9, 2000·Kidney International·L Gonzalez-MariscalJ L Reyes
Jan 5, 2001·The Journal of Membrane Biology·R MedinaC M Van Itallie
Mar 22, 2002·Biology of Reproduction·Lonnie D RussellC Michael Knudson
Dec 15, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ching-Hang WongC Yan Cheng
Nov 3, 2012·Endocrinology·Peter K NichollsCraig A Harrison
Sep 24, 2015·The Journal of Urology·Adel A ZalataOlfat G Shaker
Dec 15, 2015·The Journal of Urology·Nikhil Waingankar, Robert G Uzzo
Jun 9, 2016·Asian Journal of Andrology·Sonja GrunewaldChristopher Springsguth
Sep 7, 2016·Circulation Journal : Official Journal of the Japanese Circulation Society·Nuno Cortez-DiasFrancisco J Enguita
Dec 13, 2016·Experimental Dermatology·Meng JiangQing Sun
Aug 23, 2017·DNA and Cell Biology·Juan Ramos-TreviñoMartha P Nava-Hernández
Sep 7, 2019·Journal of Cell Science·Jisca MajoléePeter L Hordijk
Oct 1, 2019·BMC Research Notes·Honglin FengAlexandra C C Wilson
Oct 28, 2019·Ecotoxicology and Environmental Safety·Mohammad Hosein SinkakarimiAbasalt Hosseinzadeh Colagar

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
ubiquitination

Software Mentioned

Image Lab

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis