A 22 kDa protein specific for yeast mitochondrial nucleoids is an unidentified putative ribosomal protein encoded in open reading frame YGL068W

Protoplasma
H Sato, I Miyakawa

Abstract

Mitochondrial-nucleoid (mt-nucleoid) proteins of the yeast Saccharomyces cerevisiae were separated by two-dimensional gel electrophoresis. Analysis of the N-terminal amino acid sequence showed that a 22 kDa protein which is unique in the mt-nucleoid fraction is an unidentified protein encoded in the open reading frame YGL068W and shows a homology with the ribosomal protein L7/L12 of bacteria. We named this protein Mnp1p (for the mitochondrial-nucleoid protein 1). Immunoblotting of each fraction with an anti-Mnp1p antibody during the mt-nucleoid isolation showed that Mnp1p is highly concentrated in the mt-nucleoid fraction. Immunofluorescence microscopy suggested that Mnp1p is localized to mitochondria in vivo, and a significant amount of Mnp1p is associated with the mt-nucleoids. On the other hand, Northern blotting showed that a large amount of large and small mitochondrial ribosomal RNAs was not associated with the mt-nucleoids and remained in the supernatant after the isolation of mt-nucleoids. The null mutation of MNP1 led to a respiratory-deficient phenotype, but the morphology of the mt-nucleoids in the transformants carrying the null mutation was normal. These results suggest that a significant amount of Mnp1p plays a ro...Continue Reading

Citations

Jan 24, 2009·The Journal of General and Applied Microbiology·Isamu MiyakawaYu Kadowaki
Feb 2, 2011·The Journal of General and Applied Microbiology·Isamu MiyakawaHiroshi Sato
Apr 25, 2006·FEMS Yeast Research·Jozef NosekIsamu Miyakawa
Sep 2, 2016·Molecular Biology of the Cell·Michael W WoellhafJohannes M Herrmann
Oct 5, 2016·Journal of Proteome Research·Katelyn E Caslavka ZempelSteven G Clarke
Jun 15, 2010·Environmental and Molecular Mutagenesis·Timothy E Shutt, Gerald S Shadel
Nov 24, 2005·Nature Reviews. Genetics·Xin Jie Chen, Ronald A Butow
May 13, 2017·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Isamu Miyakawa

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