Oct 24, 2014

A 22,403 marker composite genetic linkage map for cassava (Manihot esculenta Crantz) derived from ten populations

BioRxiv : the Preprint Server for Biology
International Cassava Genetic Map Consortium

Abstract

Cassava ( Manihot esculenta Crantz) is a major staple crop in Africa, Asia, and South America, and its starchy roots provide nourishment for 800 million people worldwide. Although native to South America, cassava was brought to Africa approximately 400 years ago and is now widely cultivated across sub-Saharan Africa. The widespread use of clonal planting material, however, aids the spread of disease. Breeding for disease resistance and improved yield began in the 1920s and has accelerated in the last 45 years. To assist in the rapid identification of markers for pathogen resistance and crop traits, and to accelerate breeding programs, we generated a framework map for M. esculenta Crantz derived from reduced representation sequencing (genotyping-by-sequencing [GBS]). The composite 2,412 cM map integrates ten biparental maps (comprising 3,480 meioses) and organizes 22,403 genetic markers on 18 chromosomes, in agreement with the observed karyotype. The map anchors 71.9% of the draft genome assembly and 90.7% of the predicted protein-coding genes. The resulting chromosome-anchored genome sequence provides an essential framework for identification of trait markers and causal genes as well as genomics-enhanced breeding of this import...Continue Reading

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Mentioned in this Paper

Biological Markers
XYY Karyotype
Crops, Agricultural
Genome
Genes
LUC7L3
Avian Crop
Genome Assembly Sequence
Nucleic Acid Sequencing
LUC7L3 gene

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