A 250-kilodalton cellular protein is induced by progestins in two human breast cancer cell lines MCF7 and T47D

Biochemical and Biophysical Research Communications
D Chalbos, H Rochefort

Abstract

We have studied the effect of R5020, a synthetic progestin, on the biosynthesis of cellular proteins extracted from the MCF7 and T47D human breast cancer cells, using gel electrophoresis. R5020 stimulates the synthesis, as measured after [35S]-methionine labelling, and the accumulation, as shown by silver staining, of a protein of molecular weight approximately equal to 250,000. The increase of the labelled 250-kilodalton protein was rapid (3 hours) and after 3 days this protein represented approximately equal to 6% of the total cellular proteins (approximately equal to 1 microgram/150,000 cells). The induction of the 250-kilodalton protein was obtained by physiologically active concentrations of several progestins and high concentrations of 5 alpha-dihydrotestosterone but not by estradiol or dexamethasone. It was inhibited by R486 , a progestin antagonist, but not by flutamide, an androgen antagonist. These results indicate a mediation by the progesterone receptor. The 250-kilodalton protein appears to be an excellent probe to study in cell culture the mechanism of action of progestin on human cells.

References

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Citations

Jan 1, 1990·The International Journal of Biochemistry·J F SavouretE Milgrom
Sep 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·D ChalbosH Rochefort
Jun 24, 1998·Biochimica Et Biophysica Acta·R A HennigarM B Marrero
Jan 1, 1990·Annals of the New York Academy of Sciences·D ChalbosH Rochefort
Aug 28, 2012·Molecular and Cellular Endocrinology·Isabel R SchlaepferBritta M Jacobsen
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