PMID: 9664221Jul 17, 1998Paper

A 26-30 kDa developmentally-regulated tau isoform localized within nuclei of mitotic human neuroblastoma cells

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
T B Shea, C M Cressman

Abstract

Tau isoforms migrating at 46-68 and 97-115 kDa were prominent within heat-stable Triton-soluble material, and were present in lesser concentration with Triton-insoluble cytoskeletons, derived from undifferentiated SH-SY-5Y human neuroblastoma cells. Conversely, a 26-30 kDa tau isoform was enriched in the cytoskeleton and detected at relatively minor levels within cytosolic fractions. Pulse labeling with 35S-methionine indicated that this 26-30 kDa "small tau" did not represent a breakdown product of larger isoforms. Since the nucleus is retained within the Triton-insoluble cytoskeleton, additional cultures were fractionated onto sucrose to obtain purified nuclei. The vast majority of small tau was recovered within purified nuclei. Small tau was reactive with tau antibodies directed towards N-terminal, C-terminal and central epitopes, further confirming that this small isoform was not derived from proteolytic cleavage of larger tau isoforms. Small tau demonstrated alkaline phosphatase-sensitive reactivity with multiple phospho-dependent tau antibodies. Small tau was depleted within 3 days of retinoic acid-induced differentiation, suggesting that the putative function of this isoform may be obsolete following terminal differentia...Continue Reading

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Citations

Jan 12, 2016·Biomolecules·Mahmoud Bukar MainaLouise C Serpell
Mar 20, 2014·Science China. Life Sciences·Jing LuJürgen Götz
Apr 8, 2017·Acta Neuropathologica·Tong GuoDiane P Hanger
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Jun 6, 2006·Protein Expression and Purification·Daniela Volke, Ralf Hoffmann

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