A 268 bp upstream sequence mediates the circadian clock-regulated transcription of the wheat Cab-1 gene in transgenic plants

Plant Molecular Biology
E FejesF Nagy

Abstract

We previously reported that the expression of the wheat Cab-1 gene is regulated by an endogenous circadian rhythm and by the photoreceptor phytochrome both in wheat and in transgenic tobacco plants. To define regulatory elements necessary for the circadian rhythm-regulated Cab-1 gene expression, we now analysed the fluctuation of steady-state mRNA levels in a series of 5' deletion mutants in transgenic tobacco plants. We found that the expression of a deletion mutant containing 211 bp upstream sequence still exhibited circadian rhythm. Furthermore we show that an enhancer-like sequence of the Cab-1 promoter (from -357 to -90) can endow a chimaeric gene consisting of a truncated 35S promoter (from -90 to +8) and the bacterial beta-glucuronidase (GUS) gene with circadian clock-regulated gene expression. Finally we demonstrate by nuclear run-off experiments that the transcription rates of the Cab genes in wheat oscillate in a rhythmic manner, with a periodicity of approximately 24 hours. Consistent with our previous findings these results (i) indicate that the expression of the wheat Cab-1 gene is regulated mainly at the transcription level and (ii) identify a short promoter region between -211 and -90 that is responsible for the ...Continue Reading

References

Jan 1, 1989·Molecular & General Genetics : MGG·D GidoniP Dunsmuir
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E LamN H Chua
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G GiulianoA R Cashmore
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K A Marrs, L S Kaufman

❮ Previous
Next ❯

Citations

Oct 1, 1994·Plant Molecular Biology·S Hwang, D L Herrin
Jun 1, 1993·Plant Molecular Biology·B Piechulla
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F NagyE Schafer
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·C Robertson McClung
Feb 22, 2001·Annual Review of Physiology·C H Johnson
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·C J Weitz
Feb 1, 1993·Plant Molecular Biology·H J LinthorstJ F Bol
Apr 29, 1999·Chronobiology International·B Piechulla
Nov 1, 1996·Chronobiology International·J Beator, K Kloppstech
Apr 1, 1993·Current Opinion in Genetics & Development·J S Takahashi
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·M J Dowson-Day, A J Millar
Nov 20, 1998·Molecular and Cellular Biology·M L SalvadorL Bogorad

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.