PMID: 590267Nov 15, 1977

A 31P-nuclear-magnetic-resonance study of the phosphate groups in lipopolysaccharide and lipid A from Salmonella

European Journal of Biochemistry
P F MühlradtV Lehmann

Abstract

Untreated and partially deacylated lipopolysaccharides from various P- and P+ strains of Salmonella were studied with 31P nuclear magnetic resonance spectroscopy and by conventional analytical methods. The spectral signals were assigned to various phosphate groups in the lipid A moiety and in the oligosaccharide part. A signal at +2.3 ppm could be assigned to a phosphodiester linkage formed between 4-amino-4-deoxyl-L-arabinose linked via the glycosidic hydroxyl group to the 4'-phosphate group of the glucosamine disaccharide in the lipid A moiety. A strong pyrophosphate signal at +11 ppm in P- strains was identified as a pyrophosphoryl ethanolamine group at the glycosidic end of this glucosamine disaccharide unit. No evidence was found for phosphodiester or pyrophosphodiester bonds crosslinking lipopolysaccharide 'subunits'. A revised version of the lipid A structure of Salmonella is presented. By a combination of 31P nuclear magnetic resonance spectroscopy data and conventional analytical methods the extent to which the lipopolysaccharides are substituted by various phosphate groups on the lipid A and the oligosaccharide moiety could be estimated. It was thus shown that substantial heterogeneity, leading to several molecular sp...Continue Reading

References

Jun 1, 1975·European Journal of Biochemistry·C Galanos, O Lüderitz
May 1, 1970·European Journal of Biochemistry·W DrögeO Westphal
Jul 1, 1973·The Journal of Infectious Diseases·O LüderitzO Westphal
Oct 18, 1973·European Journal of Biochemistry·G HämmerlingO Lüderitz
Nov 21, 1972·Biochemistry·M P Sheetz, S I Chan
Jan 1, 1969·Annual Review of Biochemistry·M J Osborn
Jul 13, 1972·European Journal of Biochemistry·E T RietschelO Westphal
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal

Citations

Nov 1, 1978·Die Naturwissenschaften·O LüderitzJ Weckesser
Jan 1, 1981·Pharmacology & Therapeutics·O LüderitzE T Rietschel
Jul 1, 1984·Reviews of Infectious Diseases·I Mattsby-BaltzerC R Alving
May 16, 1978·European Journal of Biochemistry·V LehmannI Minner
Feb 1, 1979·European Journal of Biochemistry·J Gmeiner, S Schlecht
Jan 1, 1980·European Journal of Biochemistry·H Yamada, S Mizushima
Sep 2, 1985·European Journal of Biochemistry·N Dalla VeneziaG Michel
Apr 1, 1989·European Journal of Biochemistry·J H KraussH Mayer
Apr 15, 1996·European Journal of Biochemistry·T IidaK Tanamoto
Jun 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H Y YehD M Jacobs
Jan 1, 1984·The American Journal of Emergency Medicine·E T RietschelU Schade
Mar 9, 2010·Journal of the International Society of Sports Nutrition·Shona S CraigSteven H Zeisel
Jun 9, 2012·International Journal of Biological Macromolecules·Rodrigo V SerratoMarcello Iacomini
Oct 16, 2003·Biochimica Et Biophysica Acta·S N Chatterjee, Keya Chaudhuri
Jan 1, 1985·CRC Critical Reviews in Biochemistry·R Benz
Feb 1, 1986·Thrombosis Research·G TachiyamaT Mori
Jan 16, 1984·European Journal of Biochemistry·I Mattsby-Baltzer, C R Alving
Oct 21, 2019·European Biophysics Journal : EBJ·Y MiyagawaS Adachi
Apr 1, 1981·European Journal of Biochemistry·K W BroadyO Lüderitz
Apr 10, 2020·ACS Infectious Diseases·Toni A NagyCorrella S Detweiler

Related Concepts

Sugars
Lipid A
Lipopolysaccharides
In Vivo NMR Spectroscopy
Orthophosphate
Salmonella

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