A 3D-bioprinted scaffold with doxycycline-controlled BMP2-expressing cells for inducing bone regeneration and inhibiting bacterial infection

Bioactive Materials
Minqi WangTingting Tang

Abstract

Large bone defects face a high risk of pathogen exposure due to open wounds, which leads to high infection rates and delayed bone union. To promote successful repair of infectious bone defects, fabrication of a scaffold with dual functions of osteo-induction and bacterial inhibition is required. This study describes creation of an engineered progenitor cell line (C3H10T1/2) capable of doxycycline (DOX)-mediated release of bone morphogenetic protein-2 (BMP2). Three-dimensional bioprinting technology enabled creation of scaffolds, comprising polycaprolactone/mesoporous bioactive glass/DOX and bioink, containing these engineered cells. In vivo and in vitro experiments confirmed that the scaffold could actively secrete BMP2 to significantly promote osteoblast differentiation and induce ectopic bone formation. Additionally, the scaffold exhibited broad-spectrum antibacterial capacity, thereby ensuring the survival of embedded engineered cells when facing high risk of infection. These findings demonstrated the efficacy of this bioprinted scaffold to release BMP2 in a controlled manner and prevent the occurrence of infection; thus, showing its potential for repairing infectious bone defects.

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Citations

Mar 2, 2021·International Journal of Pharmaceutics·Katie GloverDimitrios A Lamprou
Jun 8, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Minqi WangTingting Tang
Jun 23, 2021·Essays in Biochemistry·Alessia LongoniKhoon S Lim
Aug 15, 2021·International Journal of Pharmaceutics·Salma E El-HabashyHoda M Eltaher

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Methods Mentioned

BETA
bioprinting
ELISA
transmission electron microscopy
scanning electron microscopy
X-ray
transfection
PCR

Software Mentioned

SPSS
StemEasy

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