A 3D co-culture of three human cell lines to model the inflamed intestinal mucosa for safety testing of nanomaterials

Nanotoxicology
Julia SusewindClaus-Michael Lehr

Abstract

Oral exposure to nanomaterials is a current concern, asking for innovative biological test systems to assess their safety, especially also in conditions of inflammatory disorders. Aim of this study was to develop a 3D intestinal model, consisting of Caco-2 cells and two human immune cell lines, suitable to assess nanomaterial toxicity, in either healthy or diseased conditions. Human macrophages (THP-1) and human dendritic cells (MUTZ-3) were embedded in a collagen scaffold and seeded on the apical side of transwell inserts. Caco-2 cells were seeded on top of this layer, forming a 3D model of the intestinal mucosa. Toxicity of engineered nanoparticles (NM101 TiO2, NM300 Ag, Au) was evaluated in non-inflamed and inflamed co-cultures, and also compared to non-inflamed Caco-2 monocultures. Inflammation was elicited by IL-1β, and interactions with engineered NPs were addressed by different endpoints. The 3D co-culture showed well preserved ultrastructure and significant barrier properties. Ag NPs were found to be more toxic than TiO2 or Au NPs. But once inflamed with IL-1β, the co-cultures released higher amounts of IL-8 compared to Caco-2 monocultures. However, the cytotoxicity of Ag NPs was higher in Caco-2 monocultures than in 3D...Continue Reading

References

Jan 14, 1999·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·A FrickE Wirbitzki
Mar 15, 2001·European Journal of Gastroenterology & Hepatology·N Mahmud, D G Weir
Aug 2, 2003·Current Opinion in Cell Biology·Michel Desjardins, Gareth Griffiths
Jan 11, 2005·American Journal of Respiratory Cell and Molecular Biology·Barbara M Rothen-RutishauserPeter Gehr
Jul 26, 2008·The European Respiratory Journal·E Alfaro-MorenoP H M Hoet
Jul 26, 2008·Nature Nanotechnology·Marina A Dobrovolskaia, Scott E McNeil
Jun 23, 2009·Annals of the New York Academy of Sciences·Joerg D SchulzkeMichael Fromm
Jul 21, 2009·Cell Biology and Toxicology·Brian A KoenemanDavid G Capco
Oct 2, 2009·Journal of the Royal Society, Interface·Maria Iris HermannsC James Kirkpatrick
Oct 10, 2009·Particle and Fibre Toxicology·Andrea D LehmannBarbara M Rothen-Rutishauser
Jan 26, 2010·Journal of Autoimmunity·Jonathan J PowellLaetitia C Pele
Feb 5, 2010·Environmental Health : a Global Access Science Source·Birgit K GaiserVicki Stone
Aug 28, 2010·Nanotoxicology·Tatsiana MironavaMiriam H Rafailovich
Dec 4, 2010·Diagnostic Pathology·Dirk TheegartenOlaf Anhenn
May 17, 2011·Particle and Fibre Toxicology·Jae Hyuck SungIl Je Yu
Jul 29, 2011·Particle and Fibre Toxicology·Thomas Aj KuhlbuschMichael Stintz
Oct 7, 2011·International Journal of Nanomedicine·Xiao-Dong ZhangFei-Yue Fan
Oct 18, 2011·Environmental Toxicology and Chemistry·Birgit K GaiserVicki Stone
Oct 21, 2011·Environmental Toxicology and Chemistry·Yong-Ju LeeSang-Ho Kim
Nov 22, 2011·Toxicology Letters·Christiane BeerHerman Autrup
Nov 29, 2011·Toxicology·Eleonore Fröhlich, Eva Roblegg
Dec 20, 2011·Toxicology and Applied Pharmacology·Sumit AroraKishore M Paknikar
Jan 21, 2012·Environmental Science & Technology·Alex WeirNatalie von Goetz
Feb 15, 2012·Environmental Toxicology·François PerreaultWilliam Gerson Matias
Apr 18, 2012·Toxicology and Industrial Health·Jinyang ZhangMinling Gao
Oct 23, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Birgit K GaiserVicki Stone
Nov 10, 2012·World Journal of Gastroenterology : WJG·Györgyi MűzesFerenc Sipos

❮ Previous
Next ❯

Citations

Oct 3, 2018·Critical Reviews in Food Science and Nutrition·Maria Del Carmen Ponce de León-RodríguezCaroline Laurent-Babot
Dec 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juan Carlos Carmona-HernandezClara Helena González-Correa
Apr 21, 2020·Archives of Toxicology·Roman LehnerBarbara Rothen-Rutishauser
Sep 26, 2020·Toxicology Mechanisms and Methods·Yi CaoJiamao Chen
May 10, 2020·Chemical Research in Toxicology·Angela A M KämpferRoel P F Schins

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here