A 3D scanning confocal imaging method measures pit volume and captures the role of Rac in osteoclast function

Bone
Stephanie R GoldbergMarc D Grynpas

Abstract

Modulation of Rho GTPases Rac1 and Rac2 impacts bone development, remodeling, and disease. In addition, GTPases are considered treatment targets for dysplastic and erosive bone diseases including Neurofibromatosis type 1. While it is important to understand the effects of Rac modulation on osteoclast function, two-dimensional resorption pit area measurements fall short in elucidating the volume aspect of bone resorption activity. Bone marrow from wild-type, Rac1 and Rac2 null mice was isolated from femora. Osteoclastogenesis was induced by adding M-CSF and RANKL in culture plates containing dentin slices and later stained with Picro Sirius Red to image resorption lacunae. Osteoclasts were also plated on glass cover slips and stained with phalloidin and DAPI to measure their surface area and the number of nuclei. Volumetric images were collected on a laser-scanning confocal system. Sirius Red confocal imaging provided an unambiguous, continuous definition of the pit boundary compared to reflected and transmitted light imaging. Rac1- and Rac2-deficient osteoclasts had fewer nuclei in comparison to wild-type counterparts. Rac1-deficient osteoclasts showed reduced resorption pit volume and surface area. Lacunae made by single Rac2 ...Continue Reading

References

Aug 1, 1991·Calcified Tissue International·A Boyde, S J Jones
Mar 1, 1985·Kidney International·M KayeP E Barré
Mar 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P C Dolber, M S Spach
Jan 1, 1997·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·P BallantiE Bonucci
Jun 1, 1999·European Journal of Cell Biology·S RazzoukG Cournot
Sep 1, 2000·Science·S L Teitelbaum
Nov 19, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Akira FukudaSakae Tanaka
Jun 15, 2006·Journal of Cell Science·Ann P WheelerAnne J Ridley
Jul 31, 2007·Odontology·Naoyuki TakahashiHidehiro Ozawa
Oct 24, 2007·The Journal of Cell Biology·Chun Xiang SunMichael Glogauer
Mar 6, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rama GarimellaH Clarke Anderson
Aug 23, 2008·Nature Reviews. Molecular Cell Biology·Sarah J Heasman, Anne J Ridley
Dec 10, 2008·Chembiochem : a European Journal of Chemical Biology·Dafna GeblingerLia Addadi
Aug 4, 2009·Calcified Tissue International·Yongqiang WangMichael Glogauer
Oct 1, 2009·Ageing Research Reviews·Deborah V Novack, Roberta Faccio
Aug 5, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J K R S MagalhaesM Glogauer
Nov 27, 2010·Calcified Tissue International·Takashi ItokowaKarl Insogna
Dec 24, 2010·Cell Adhesion & Migration·Désirée Spiering, Louis Hodgson
Jul 22, 2011·Small GTPases·Cecile ItzsteinMichael J Rogers
Jul 27, 2011·Journal of Immunological Methods·Silvia D Potenziani PradellaAnthony J Janckila

❮ Previous
Next ❯

Citations

Jul 25, 2014·Journal of Cellular Physiology·Jiro TakitoMasanori Nakamura
Jan 31, 2014·Journal of Tissue Engineering and Regenerative Medicine·Rainer Detsch, Aldo R Boccaccini
Jun 3, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jesse L MattesonJoshua J Stapleton
Mar 13, 2014·Small GTPases·Heiani TouaitahuataVirginie Vives
Nov 26, 2013·The American Journal of Pathology·Corneliu SimaMichael Glogauer
Apr 28, 2018·Journal of Tissue Engineering and Regenerative Medicine·Anwarul HasanHany E Marei
Nov 5, 2016·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ee Min TanEu-Leong Yong
Apr 22, 2018·Stem Cell Research & Therapy·Alessandro PirosaRocky S Tuan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Yongqiang WangMichael Glogauer
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Keiko SuzukiHisashi Shinoda
© 2021 Meta ULC. All rights reserved