A 40-Month Follow-Up of Ebola Virus Disease Survivors in Guinea (PostEbogui) Reveals Long-Term Detection of Ebola Viral Ribonucleic Acid in Semen and Breast Milk

Open Forum Infectious Diseases
Alpha Kabinet KeitaPostebogui Study Group

Abstract

With the increasing frequency and impact of Ebola virus disease (EVD) outbreaks illustrated by recent epidemics, a good understanding of the extent of viral persistance or ribonucleic acid (RNA) detection in body fluids from survivors is urgently needed. Ebola viral RNA shedding was studied with molecular assays in semen (n = 1368), urine (n = 1875), cervicovaginal fluid (n = 549), saliva (n = 900), breast milk (n = 168), and feces (n = 558) from EVD survivors in Guinea (PostEbogui cohort, n = 802) at a regular base period until 40 months after inclusion. Twenty-seven of 277 (9.8%) male survivors tested positive for Ebola RNA in at least 1 semen sample. The probability of remaining positive for Ebola RNA in semen was estimated at 93.02% and 60.12% after 3 and 6 months. Viral RNA in semen was more frequent in patients with eye pain (P = .036), joint pain (P = .047), and higher antibody levels to Ebola virus antigens (nucleoprotein [P = .001], glycoprotein [P = .05], and viral protein-40 [P = .05]). Ebola RNA was only rarely detected in the following body fluids from EVD survivors: saliva (1 of 454), urine (2 of 593), breast milk (2 of 168), cervicovaginal secretions (0 of 273), and feces (0 of 330). Ribonucleic acid was detected...Continue Reading

Citations

Jul 28, 2020·Nature Communications·Aurélie WiedemannUNKNOWN PostEboGui Study Group
Oct 29, 2020·Annals of the New York Academy of Sciences·Melisa Medina-RiveraSaurabh Mehta
Mar 24, 2021·Emerging Infectious Diseases·Courtney M BozmanLisa E Hensley
Sep 17, 2021·Nature·Alpha Kabinet KeitaN' Faly Magassouba

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Methods Mentioned

BETA
PCR
reverse-transcription PCR
Assay

Software Mentioned

BioFire
RealStar Screen
RealStar

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