A 5' extended IFN-stimulating response element is crucial for IFN-gamma-induced tripartite motif 22 expression via interaction with IFN regulatory factor-1

The Journal of Immunology : Official Journal of the American Association of Immunologists
Bo GaoSidong Xiong

Abstract

Interferon-gamma is crucial for the noncytopathic clearance of hepatitis B virus. In our previous study, we demonstrated that an IFN-gamma-inducible molecule, tripartite motif (TRIM) 22, played an important role in antiviral immunity against hepatitis B virus. However, the molecular mechanism of TRIM22 induction by IFN-gamma is still unclear. In this study, we identified a novel cis-element termed 5' extended IFN-stimulating response element (5' eISRE) that was crucial for IFN-gamma inducibility of TRIM22 through transfection assays with luciferase reporter constructs and EMSAs. The 5' eISRE consists of an ISRE-like motif (ACTTTCGTTTCTC) and a 6-bp sequence (AATTTA) upstream of it, and all three thymine triplets of this cis-element (AATTTAACTTTCGTTTCTC) were revealed to contribute to the IFN-gamma inducibility of TRIM22 by site-directed mutagenesis. Further studies showed that upon IFN-gamma stimulation, the 5' eISRE could be bound by IFN regulatory factor-1 (IRF-1), but not by STAT1, as demonstrated by supershift analysis and an ELISA-based transcription factor assay. Moreover, overexpression of IRF-1 significantly induced TRIM22 expression, whereas silencing of IRF-1 with specific short interference RNA abolished IFN-gamma-in...Continue Reading

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Citations

May 11, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Bo GaoSidong Xiong
Jun 1, 2012·Molecular Biology International·Clayton J HattlmannStephen D Barr
Aug 14, 2019·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yuanyuan YangSidong Xiong
Jun 5, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bo GaoSidong Xiong
Jul 28, 2021·Molecular Neurobiology·Shiek S S J AhmedRam Murugesan
Aug 28, 2021·Cells·Isabel PaganiElisa Vicenzi

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