PMID: 19912882Oct 1, 1992Paper

A 5'-flanking region of the bovine tyrosine hydroxylase gene is involved in cell-specific expression, activation of gene transcription by phorbol ester, and transactivation by c-Fos and c-Jun

Molecular and Cellular Neurosciences
A GocM K Stachowiak

Abstract

Tyrosine hydroxylase (TH) is expressed specifically in catecholaminergic cells and is transcriptionally activated via a protein kinase C (PKC)-dependent pathway. To identify regulatory regions of the TH gene, transfected neural crest-derived catecholaminergic cells [human neuroblastoma SH-SY5Y and bovine adrenal medullary (BAM) cells] or glia-derived SF-763 cells were used to analyze expression of a chloramphenicol acetyltransferase (CAT) reporter gene under the control of 5'-flanking sequences of the bovine TH gene. Plasmid pTH(-245/+21)CAT was constructed by fusing the -245 to +21 region of the TH gene to CAT sequences in the promoterless pCAT basic plasmid. Cells transfected with pTH(-245/+21)CAT expressed CAT activity at levels 8- to 100-fold higher than those of cells transfected with pCAT basic. In SH-SY5Y or BAM cells, pTH(-245/+21)CAT supported the expression of CAT at levels similar to or higher than that supported by the tissue nonspecific viral SV40 promoter/enhancer. In glia-like cells, CAT expression from pTH(-245/+2 1)CAT was about 13-fold lower than that under the control of the SV40 promoter. Incubation of neuroblastoma cells with the active phorbol ester, phorbol 12-myristate 13-acetate (PMA), increased express...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G TeitelmanD J Reis
Oct 1, 1979·The Biochemical Journal·B B KaplanA E Gioio
Oct 1, 1991·Brain Research. Molecular Brain Research·Z M HuangW D Wicks
Jun 1, 1990·Brain Research. Molecular Brain Research·D Fader, E J Lewis
Jan 1, 1986·Journal of Neuroscience Research·M K StachowiakB B Kaplan
Aug 18, 1988·Nature·W W LamphI M Verma
Dec 22, 1989·Science·J L SonnenbergT Curran
May 1, 1989·Journal of Neuroscience Research·S R D'MelloB B Kaplan
Nov 1, 1989·Journal of Neurochemistry·F CambiD Chikaraishi
Sep 1, 1987·Molecular and Cellular Biology·E J Lewis, D M Chikaraishi
Feb 1, 1987·Journal of Neuroscience Methods·S P Wilson
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·D M Chuang, E Costa
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J MalletD Samolyk
Apr 1, 1983·Journal of Neuroscience Methods·J C WaymireJ W Haycock

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Citations

Jul 1, 1995·Brain Research. Molecular Brain Research·M K McMillianK R Pennypacker

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