PMID: 7539134May 23, 1995Paper

A 5'-upstream region of a bovine keratin 6 gene confers tissue-specific expression and hyperproliferation-related induction in transgenic mice

Proceedings of the National Academy of Sciences of the United States of America
A RamírezJ L Jorcano

Abstract

Keratins, the constituents of epithelial intermediate filaments, are precisely regulated in a tissue- and development-specific manner, although little is known about the molecular mechanisms underlying this regulation. The expression pattern of keratin 6 is particularly complex, since besides being constitutively expressed in hair follicles and in suprabasal cells of a variety of internal stratified epithelia, it is induced in epidermis in both natural and artificially caused hyperproliferative situations. Therefore, the regulatory sequences controlling keratin 6 gene activity are particularly suitable for target gene expression in a tissue-specific manner. More interestingly, they can be skin-induced in transgenic animals or in gene therapy protocols, particularly those addressing epidermal hyperproliferative disorders. To delimit the regions containing these regulatory elements, different parts of the bovine keratin 6 gene linked to a beta-galactosidase reporter gene have been assayed in transgenic mice. A 9-kbp fragment from the 5' upstream region was able to provide both suprabasal tissue-specific and inducible reporter expression.

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Citations

Aug 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Byrne
Jan 10, 2006·Carcinogenesis·David J FeithLisa M Shantz
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·K Takahashi, P A Coulombe
Oct 22, 2003·The Journal of Cell Biology·Pauline Wong, Pierre A Coulombe
Nov 4, 2000·Expert Opinion on Investigational Drugs·W Pfützner, J C Vogel
Feb 17, 2001·The Journal of Investigative Dermatology·M KomineM Blumenberg
Dec 17, 2003·The Journal of Investigative Dermatology·Zhiling WangPierre A Coulombe
Dec 15, 2010·The Journal of Investigative Dermatology·Marta GarcíaMarcela Del Rio
Oct 4, 2008·The American Journal of Pathology·Lisa J McCawleyLynn M Matrisian
Apr 3, 2012·Genesis : the Journal of Genetics and Development·Marlon R Schneider
Jun 1, 2014·Actas dermo-sifiliográficas·F LarcherM Quintanilla
Sep 19, 2015·Cancer Growth and Metastasis·Shannon L NowotarskiLisa M Shantz
Jul 18, 2002·The Journal of Biological Chemistry·Mirentxu SantosJosé L Jorcano
Jul 18, 1997·Biochemical and Biophysical Research Communications·P A Coulombe

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