PMID: 9527522Apr 4, 1998Paper

A 5.0-kD heparin fraction systemically suppresses VEGF165-mediated angiogenesis

International Journal of Microcirculation, Clinical and Experimental
Klas Norrby, P Ostergaard

Abstract

The systemic effect of heparin fractions with mean molecular masses of 2.5, 5.0 and 16.4 kD on angiogenesis induced by vascular endothelial growth factor isoform 165 was studied using the truly quantitative rat mesenteric-window angiogenesis assay. The angiogenic treatment with 5 ml of VEGF165 at 480 pM was given intraperitoneally on days 0-4 and heparin fractions were given subcutaneously on days 0-13; animals were sacrificed on day 14. As the overlaps between the molecular mass distributions of the three fractions were relatively small, they essentially represent three different populations of heparin molecules. The doses of the heparins given were equal in terms of weight, but different in terms of the number of molecules and biologic activity. Angiogenesis was assessed in terms of vascularized area (VA), a measurement of microvascular spatial extension, and microvascular length (MVL), a measurement of microvascular density, using technically independent variables and image analysis. The total microvascular length was computed from VA x MVL. Treatment with the 5.0-kD fraction suppressed angiogenesis significantly in statistical terms compared with treatment with 2.5- and 16.4-kD heparins and the saline in controls. Interesti...Continue Reading

Citations

Mar 8, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Klas Norrby
Feb 14, 2012·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Shaker A Mousa
Jun 8, 2018·Journal of Thrombosis and Haemostasis : JTH·J M WatJ C Kingdom
Nov 26, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Klas Norrby, Arvid Nordenhem

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