PMID: 3367095May 1, 1988Paper

A 52-kD protein is a novel component of the SS-A/Ro antigenic particle

The Journal of Experimental Medicine
E Ben-ChetritE M Tan


Anti-SS-A/Ro autoantibodies are found in the sera of patients with Sjogren's syndrome (SS) and SLE. In the course of analyzing 61 SS patients for their autoantibody profiles, we found that 42 were positive for anti-SS-A by double diffusion in agarose and demonstrated precipitin lines identical to that produced by a prototype anti-SS-A serum. Further analysis of these SS-A antibody-positive sera by Western blotting of cell extracts revealed that 21 sera reacted with two proteins of 60 and 52 kD, 13 sera reacted with 52-kD protein, two detected only 60 kD, while six were nonreactive. Affinity-purified anti-60-kD and anti-52-kD antibodies reacted exclusively with their corresponding antigens. Partial proteolysis of these proteins did not reveal common degradation fragments. Thus the 52- and 60-kD proteins were found to be antigenically and apparently structurally distinct from each other. They were also distinct from 48-kD SS-B/La protein. In immunoprecipitation using labeled cell extracts, affinity-purified anti-52-kD antibodies brought down the 52-kD protein as well as the 60-kD band. In [32P]orthophosphate-labeled HeLa cell extract both antibodies precipitated the same spectrum of small RNAs (hYl-5). In indirect immunofluoresce...Continue Reading


Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R Lerner, J A Steitz
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 1, 1975·The Journal of Clinical Investigation·M A Alspaugh, E M Tan
Jan 1, 1986·The American Journal of Medicine·C L Christian, K B Elkon
May 1, 1986·Arthritis and Rheumatism·R I FoxF V Howell
Feb 1, 1985·The Journal of Clinical Investigation·W H ReevesH G Kunkel
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A M Francoeur, M B Mathews
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·Sandra L Wolin, J A Steitz
Nov 11, 1983·Journal of Immunological Methods·H H GuldnerF A Bautz
Feb 1, 1984·Arthritis and Rheumatism·C E HarmonE M Tan
Jul 6, 1984·Journal of Immunological Methods·T S LieuE M Tan


Feb 20, 1999·Arthritis and Rheumatism·R H ScofieldJ B Harley
Dec 1, 1990·Arthritis and Rheumatism·Y ItohM Reichlin
Nov 1, 1993·Arthritis and Rheumatism·G A McCartyM Reichlin
Sep 1, 1994·Arthritis and Rheumatism·E W St ClairM R Saitta
Jul 1, 1995·Arthritis and Rheumatism·T KatoK Yamamoto
Apr 12, 2002·Journal of Clinical Laboratory Analysis·M J FritzlerTheophany Eystathioy
Jan 1, 1991·Rheumatology International·P J Maddison
Jan 1, 1990·Molecular Biology Reports·G J PruijnW J Van Venrooij
Jan 1, 1990·Molecular Biology Reports·R L SlobbeW J Van Venrooij
Sep 1, 1992·Molecular Biology Reports·G J PruijnW J Van Venrooij
Jun 1, 1992·Molecular Biology Reports·S Whittingham
Mar 1, 1990·Clinical Rheumatology·D G WilliamsR N Maini
May 1, 1989·Rheumatic Diseases Clinics of North America·M PetriM C Hochberg
Mar 1, 1990·Clinical Rheumatology·H M Moutsopoulos, L V Zerva
Nov 15, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·May Penrad-MobayedJean-Antoine Lepesant
Dec 21, 2007·Clinical Reviews in Allergy & Immunology·Julie H LinVictoria P Werth
Nov 10, 2007·Clinical Reviews in Allergy & Immunology·J G Routsias, A G Tzioufas
Jul 26, 1989·Journal of Immunological Methods·D H WuS Bombardieri
Sep 13, 1991·Journal of Immunological Methods·E L TreadwellA Volkman
Jan 22, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·J G RoutsiasH M Moutsopoulos
Jul 5, 2002·Journal of Molecular Biology·Michael A FourauxG J Pruijn
May 14, 1999·Immunology Today·M Wahren-HerleniusD A Isenberg
May 15, 2002·Journal of Dermatological Science·Takashi YoshimasuFukumi Furukawa
Jun 29, 2001·Trends in Cardiovascular Medicine·M Boutjdir
Oct 7, 2008·Nature Reviews. Immunology·Keiko OzatoHerbert C Morse
Mar 21, 2002·The British Journal of Dermatology·T P MillardJ A McGrath
Apr 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anthony H KeebleLeo C James
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S L DeutscherJ D Keene
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D P SilverD Baltimore
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C O'BrienSandra L Wolin
Jan 1, 1990·Journal of Immunoassay·C B Rothschild, B A Kilpatrick
Oct 11, 1991·Nucleic Acids Research·G J PruijnW J Van Venrooij
Feb 1, 1994·Scandinavian Journal of Immunology·M WahrenI Pettersson
Mar 1, 1995·Scandinavian Journal of Immunology·S C HuangJ B Harley
Jun 1, 1992·Australian and New Zealand Journal of Medicine·A Sturgess
Oct 31, 1998·Immunological Reviews·J McCluskeyTom P Gordon
Oct 1, 1992·American Journal of Reproductive Immunology : AJRI·E Ben-Chetrit
Feb 24, 2004·The British Journal of Ophthalmology·E TokerH Direskeneli
Nov 5, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J C LabbéL A Rokeach
Jan 11, 2013·Clinical & Developmental Immunology·Ryusuke YoshimiYoshiaki Ishigatsubo
Jun 16, 2012·International Journal of Rheumatology·Ryusuke YoshimiKeiko Ozato
Apr 1, 1989·The Journal of Clinical Investigation·M D RaderM Reichlin
May 1, 1990·The Journal of Clinical Investigation·D P McCauliffeM Reichlin
Jan 1, 1991·The Journal of Clinical Investigation·E K ChanE M Tan
Jan 6, 2011·BMC Biochemistry·Alak K KarJoseph Sodroski
Mar 10, 2009·Journal of Autoimmune Diseases·Jennifer C ParkerChristopher C Bunn
May 2, 2012·Arthritis Research & Therapy·Aurélie Ambrosi, M Wahren-Herlenius
Mar 24, 2004·Paediatric Drugs·Lela A Lee
Feb 11, 2012·Journal of Neurogastroenterology and Motility·Jackie D WoodWilliam E Whitehead
Mar 21, 1998·Lancet·E Ben-Chetrit, M Levy
Aug 10, 2011·EMBO Molecular Medicine·Taro Kawai, Shizuo Akira
Mar 1, 1990·Clinical Rheumatology·A van DamT Cuypers
Jul 11, 2001·Journal of Molecular and Cellular Cardiology·Y QuM Boutjdir
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·V J LaMorteR M Evans
May 9, 2012·ISRN Rheumatology·João Pedro FerreiraCarlos Vasconcelos
Apr 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Leo C JamesJohn Trowsdale
Aug 1, 1993·Molecular Biology Reports·W J Van VenrooijG J Pruijn
Jul 11, 2002·Oral Diseases·Roland JonssonPeter Szodoray
Oct 1, 2013·Rheumatology International·Pei-Hsuan ChenBor-Luen Chiang
Oct 1, 1996·Leukemia & Lymphoma·Z M MuK S Chang
Apr 1, 2006·Critical Reviews in Clinical Laboratory Sciences·J G RoutsiasA G Tzioufas
Jan 1, 1990·Autoimmunity·D E ByersM Reichlin
Jan 1, 1994·Autoimmunity·Tom P GordonJ McCluskey
Oct 28, 2015·Biopolymers·Inger Ødum NielsenGunnar Houen
Mar 10, 2012·Journal of Autoimmunity·V Oke, M Wahren-Herlenius
Nov 15, 2011·Seminars in Arthritis and Rheumatism·Isabelle MarieFabienne Jouen
Sep 30, 2010·Biochemical and Biophysical Research Communications·Riho KurataHidetoshi Inoko
Dec 25, 2009·Journal of Autoimmunity·Markus H HoffmannG Steiner
Dec 18, 2015·Arthritis Research & Therapy·Maria InfantinoNicola Bizzaro
Nov 6, 2008·Rheumatic Diseases Clinics of North America·Beth L CobbKathy L Moser
May 31, 2008·Journal of Autoimmunity·Joanne H ReedTom P Gordon
Mar 26, 2008·Biochemical and Biophysical Research Communications·Tadanori YamochiChikao Morimoto
Jul 15, 2005·Annals of the New York Academy of Sciences·Petra EissfellerArno Kromminga
Sep 1, 2004·Journal of Separation Science·Natalia SaccodossiRita R Stumpo
Oct 19, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·William A McEwanLeo C James
Jul 16, 2010·European Journal of Clinical Investigation·J G Routsias, A G Tzioufas
Aug 9, 2011·Wiley Interdisciplinary Reviews. RNA·Soyeong Sim, Sandra L Wolin
Mar 8, 2013·Journal of Internal Medicine·V Oke, M Wahren-Herlenius
Apr 1, 1995·Seminars in Arthritis and Rheumatism·Carlos A von Mühlen, E M Tan
Aug 1, 1989·Journal of Autoimmunity·D P McCauliffeJ D Capra
Apr 1, 1990·Baillière's Clinical Rheumatology·L A Lee
Apr 1, 1990·Baillière's Clinical Rheumatology·J Buyon
Aug 13, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Umesh S DeshmukhShu Man Fu
May 15, 2007·Rheumatic Diseases Clinics of North America·Peter M IzmirlyJ Buyon
Oct 17, 2014·Biology of the Cell·Dhanendra Tomar, Rajesh Singh
Jan 1, 1995·Scandinavian Journal of Rheumatology·A López-LunaR Herrera-Esparza
Nov 1, 1995·American Journal of Obstetrics and Gynecology·J CopelC S Kleinman
Dec 7, 2010·Current Opinion in Immunology·Finlay W McNabAnne O'Garra
Aug 9, 2012·Journal of Autoimmunity·Georgia ArentzTom P Gordon
Jun 30, 2012·Autoimmunity Reviews·Sonal MehraM J Fritzler
Apr 30, 2016·International Journal of Rheumatic Diseases·Chingching FoocharoenRatanavadee Nanagara
May 5, 2016·Digestive Diseases and Sciences·Jackie D Wood
Dec 16, 2011·Lupus·E AntigaP Fabbri
Aug 10, 2000·Anais Da Academia Brasileira De Ciências·S Garcia, A C Campos-de-Carvalho
Oct 1, 1993·Lupus·L A LeeW L Weston
Aug 20, 2016·Lupus·M J Fritzler, E K L Chan
Jul 25, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kenji TatedaHiroshi Matsumoto
Sep 16, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Robert M Clancy, J Buyon
Feb 2, 2018·Modern Rheumatology·Reikou KamiyamaHideaki Nakajima
Aug 24, 1999·The Journal of Biological Chemistry·S A RutjesG J Pruijn
May 16, 2019·Critical Reviews in Biochemistry and Molecular Biology·Marco Boccitto, Sandra L Wolin
Jul 30, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Wei WeiGuoyao Tang
Jan 1, 1991·Springer Seminars in Immunopathology·R I FoxP Pisa
Nov 2, 2002·Scandinavian Journal of Immunology·R H ScofieldB T Kurien
Jan 1, 1993·Clinical and Experimental Immunology·J KarshA I Lazarovits
Nov 1, 1993·Clinical and Experimental Immunology·B BozicW J Van Venrooij
Jun 1, 1995·Clinical and Experimental Immunology·E D SilvermanK B Elkon
Mar 1, 1994·Clinical and Experimental Immunology·M B FrankV McCubbin
Feb 5, 1999·Clinical and Experimental Immunology·A HalseR Jonsson
Feb 18, 2017·International Journal of Rheumatology·Ana Alonso-LarrugaYvelise Barrios
May 1, 1992·Clinical and Experimental Immunology·M F GonzalezB Sanchez
Oct 1, 1991·Clinical and Experimental Immunology·R L SlobbeW J Van Venrooij
Mar 3, 2020·Frontiers in Immunology·Yosuke KunishitaHideaki Nakajima
Jan 1, 1990·Molecular Biology Reports·E K ChanE M Tan
May 1, 1990·Arthritis and Rheumatism·J Buyon, R J Winchester
Mar 1, 1993·Clinical and Experimental Immunology·J G RoutsiasH M Moutsopoulos
Jan 1, 1995·Clinical and Experimental Immunology·C BoulangerG Boire
Mar 1, 1990·Clinical Rheumatology·P J Maddison
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tomonori IshiiChikao Morimoto
Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M RestivoM Boutjdir
Dec 25, 2018·Frontiers in Immunology·Athanasios GkoutzourelasLazaros I Sakkas
Sep 27, 2019·Frontiers in Immunology·Stian FossJan Terje Andersen
Jan 1, 1995·Clinical and Experimental Immunology·M Reichlin
Mar 16, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nagako AkenoYaron Tomer
Jul 1, 1995·Clinical and Experimental Immunology·C H VeldhovenR J Smeenk
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryusuke YoshimiKeiko Ozato
Sep 1, 1995·Arthritis and Rheumatism·Y NiimiJ C Bystryn
Sep 20, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nina Judith HosNirmal Robinson
May 11, 2021·European Journal of Internal Medicine·Paul DeckerRoland Jaussaud

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.