A 5.5-kb enhancer is both necessary and sufficient for regulation of Wnt-1 transcription in vivo

Developmental Biology
P S DanielianA P McMahon

Abstract

Wnt-1 encodes a secreted signaling molecule which is required for development of the midbrain and anterior hindbrain in the mouse. Wnt-1 expression is initiated early in the development of the central nervous system in a region predicted to give rise to the midbrain. Later in development Wnt-1 expression is restricted to regions of the forebrain, midbrain, and spinal cord. Previous studies identified a 5.5-kb enhancer in the Wnt-1 locus which is sufficient to activate transcription of a reporter gene in a pattern very similar to that of the endogenous Wnt-1 gene. Here we have assessed if this enhancer is an important component of the endogenous regulatory sequences of Wnt-1 by gene targeting and by testing if it is able to express Wnt-1 in a pattern which is sufficient to rescue the phenotype of loss of Wnt-1. Our results show that the 5.5-kb enhancer is both necessary and sufficient for Wnt-1 expression in vivo.

References

Nov 29, 1991·Cell·K R ThomasM R Capecchi
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A JakobovitsG R Martin
Nov 1, 1995·Genes & Development·I MatsuoS Aizawa
Mar 30, 1995·Nature·W Shawlot, R R Behringer
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·K ArakiP Vassalli
Jul 29, 1994·Cell·R Krumlauf
Feb 11, 1993·Nature·J M SlackC F Graham
Jan 1, 1994·Genes & Development·S TakadaA P McMahon
Nov 15, 1996·Science·A Lumsden, R Krumlauf
Oct 14, 1996·The Journal of Comparative Neurology·G S MastickS S Easter

❮ Previous
Next ❯

Citations

Feb 20, 2003·Developmental Biology·Arne C LekvenRandall T Moon
Jul 20, 2005·Development, Growth & Differentiation·Qi TianXiang Lu
Feb 9, 2010·American Journal of Respiratory Cell and Molecular Biology·Aliete LangsdorfXingbin Ai
Feb 15, 2013·PLoS Genetics·Maria Di BonitoMichèle Studer
Jul 14, 2009·PloS One·J Susie ZoltewiczAndrew S Peterson
Mar 25, 2014·Nature Methods·Diane E DickelLen A Pennacchio
Dec 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuanyi FengChristopher A Walsh
Oct 1, 2016·Epigenetics & Chromatin·David H TaylorPaul D Soloway
May 25, 2007·Genesis : the Journal of Genetics and Development·Ke WeiFabian Chen
Mar 3, 2019·Development Genes and Evolution·Arne C LekvenAlvin T Yeh
Dec 10, 1999·Experimental Cell Research·M UusitaloS Vainio
Dec 3, 2008·Critical Reviews in Oncology/hematology·Cherry Ee Lin NgMotomi Osato

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.