A 57-bp deletion in the ovine KAP6-1 gene affects wool fibre diameter

Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie
H ZhouJ G H Hickford

Abstract

High glycine-tyrosine keratin-associated proteins (HGT-KAPs) are predominantly present in the orthocortex of wool fibres. They vary in abundance in different wools and have been implicated in regulating wool fibre properties, but little is known about the functional roles of these proteins in the fibre matrix. In this study, we used polymerase chain reaction--single-strand conformational polymorphism (PCR-SSCP) analysis to screen for variation in a gene encoding the ovine HGT-KAP6-1 protein. We identified three gene variants (A, B and C). Variants A and B were similar to each other, with only three nucleotide differences occurring downstream of the coding sequence. However, variant C had a 57-bp deletion that would notionally result in a loss of 19 amino acids in the protein. The presence of C was found to be associated with an increase in mean fibre diameter (MFD), fibre diameter standard deviation (FDSD), coefficient of variation of fibre diameter (CVFD) and prickle factor (percentage of fibres over 30 microns; PF). Sheep of genotype BC produced wool of greater MFD, FDSD and PF than sheep of genotypes AA, AB and BB. The CVFD was greater in the BC sheep than the AB sheep. The results suggest that variation in ovine KRTAP6-1 af...Continue Reading

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Citations

May 31, 2016·Genes·Hua GongJon G H Hickford
Apr 6, 2016·Scientific Reports·Huitong ZhouJon G H Hickford
Aug 13, 2017·Tropical Animal Health and Production·Fatemeh EbrahimiAyoub Farhadi
Jan 10, 2019·Animals : an Open Access Journal From MDPI·Jiqing WangJon G H Hickford
Apr 17, 2019·Animals : an Open Access Journal From MDPI·Lingrong BaiJon G H Hickford
Dec 7, 2019·Archives Animal Breeding·Wenhao LiJon G H Hickford
Apr 7, 2020·Animal Biotechnology·Ahmed Mohamed SallamEssam Mohamed Al-Bitar
Jun 20, 2020·Tropical Animal Health and Production·Farman UllahJon G H Hickford

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